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- 2026年03月17日
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- 相关疾病：
正常
- ATCC Number：
CL-101™
- 运输方式：
冻存运输
- 物种来源：
猪
- 是否是肿瘤细胞：
0
- 年限：
3 to 4 weeks
- 品系：
Hampshire
- 细胞形态：
上皮样
- 库存：
大量
- 器官来源：
肾脏
| Designations: | LLC-PK1 | ||
| Depositors: | Eli Lilly & Co. | ||
| Biosafety Level: | 1 | ||
| Shipped: | frozen | ||
| Medium & Serum: | See Propagation | ||
| Organism: | Sus scrofa | ||
| Morphology: | epithelial |
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| Source: | Organ: kidney Strain: Hampshire Disease: normal |
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| Cellular Products: | plasminogen activator | ||
| Permits/Forms: | In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location. | ||
| Age: | 3 to 4 weeks | ||
| Gender: | male | ||
| Propagation: | ATCC complete growth medium: The base medium for this cell line is Medium 199 containing 1.5 g/L to 2.2 g/L sodium bicarbonate. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 3%. Temperature: 37.0°C Atmosphere: air, 95%; carbon dioxide (CO2), 5% |
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| Subculturing: | Subcultivation Ratio: A subcultivation ratio of 1:3 to 1:8 is recommended Medium Renewal: Twice per week Protocol: Volumes used in this protocol are for 75 sq cm flasks; proportionally reduce or increase amount of dissociation medium for culture vessels of other sizes.
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| Preservation: | culture medium 95%; DMSO, 5% | ||
| Related Products: | recommended serum:ATCC 30-2020 formerly distributed as:ATCC CRL-1392 |
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| References: | 3520: Hull RN, Huseby RM. Enhanced production of plasminogen activator. US Patent 3,904,480 dated Sep 9 1975 22659: Perantoni A, Berman JJ. Properties of Wilms' tumor line (TuWi) and pig kidney line (LLC-PK1) typical of normal kidney tubular epithelium. In Vitro 15: 446-454, 1979. PubMed: 225262 28301: Loffler S, et al. CD9, a tetraspan transmembrane protein, renders cells susceptible to canine distemper virus. J. Virol. 71: 42-49, 1997. PubMed: 8985321 |
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文献和实验我和师兄是从04年初就开始用LLC-PK1细胞系筛选多种类型的、数量极多的各种可能有用的新药，摸索发现消化液的配方为0.25％的胰酶+0.02%EDTA 就行了，要配在PBS中，如果能加Hepes那就更保险了（有一次实验室Hepes用光了，配液时没加Hepes，照样没问题） 消化步骤：吸走培液后最好用PBS过一遍－>50ML培养瓶加2ML在37ºC预热消化液－>37ºC温箱放置1Min－>显微镜下观察，细胞松散开，但尚未脱落时，及时吸走消化液－>必要时可在吸走消化液的情况下，继续放置一会
LLC-RK1 Cell Screening Test for Nephrotoxicity
In this test, kidney-derived cells are cultured in the presence of test compounds whose cytotoxicity is then determined by the Neutral Red method, and serves as an indicator of potential nephrotoxicity (1 ). Healthy LLC-RK1 cells
This chapter describes method for evaluation of nanomaterial cytotoxicity by examining effects on porcine kidney (LLC-PK1) and human cancerous liver cells (Hep G2). Several studies indicate that many nanoparticles are cleared from the body
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