immunoglobulin; monoclonal antibody; against H-2 K k; cytotoxic
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Comments:
Mice were immunized with lymphocytes from C3H mice. Spleen cells were fused with NS-1 myeloma cells. The antibody cross-reacts with H-2p, H-2r and H-2Kq. This line was obtained from the same fusion as ATCC HB-25 (16-3-1N) and demonstrates the same pattern of reactivity on a test panel as ATCC HB-25. Tested and found negative for ectromelia virus (mousepox).
ATCC complete growth medium: The base medium for this cell line is ATCC Hybri-Care Medium, Catalog No. 46-X. Hybri-Care Medium is supplied as a powder and should be reconstituted in 1 L cell-culture-grade water. To make the complete growth medium, add the following components to the base medium:
fetal bovine serum to a final concentration of 10%
1.5 g/L sodium bicarbonate for use with 5% CO2 in air atmosphere.
Subculturing:
Medium Renewal: Every 2 to 3 days Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 2 X 10 exp5 cells/ml and maintain between 1 X 10 exp5 and 1 X 10 exp6 cells/ml.
Related Products:
Recommended medium (without the additional supplements or serum described under ATCC Medium):ATCC 46-X
References:
905: Ozato K, et al. Hybridoma cell lines secreting monoclonal antibodies to mouse H-2 and Ia antigens. J. Immunol. 124: 533-540, 1980. PubMed: 7188699