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- 库存：
大量
- 相关疾病：
正常
- 运输方式：
冻存运输
- ATCC Number：
CRL-8798™
- 生长状态：
贴壁生长
- 细胞形态：
上皮样
- 组织来源：
epithelium
- 器官来源：
乳房
- 物种来源：
人
- 是否是肿瘤细胞：
0
- 年限：
21 years
- 细胞类型：
其他细胞类型
| Designations: | 184A1 | ||
| Depositors: | The United States of America | ||
| Biosafety Level: | 1 | ||
| Shipped: | frozen | ||
| Medium & Serum: | See Propagation | ||
| Growth Properties: | adherent | ||
| Organism: | Homo sapiens | ||
| Morphology: | epithelial |
||
| Source: | Organ: mammary gland; breast Tissue: epithelium Disease: normal Cell Type: chemically transformed |
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| Permits/Forms: | In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location. | ||
| DNA Profile (STR): | Amelogenin: X CSF1PO: 10,11 D13S317: 11 D16S539: 11,12 D5S818: 11,13 D7S820: 9,11 THO1: 9.3 TPOX: 11 vWA: 18,19 |
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| Cytogenetic Analysis: | 45, XX | ||
| Age: | 21 years | ||
| Gender: | female | ||
| Comments: | The 184A1 cell line was established from normal mammarytissue obtained from a normal reduction mammoplasty. Cells derived from the tissue were exposed to benzo(a)pyrene, and a transformed line was established. The line appears to be immortal, but is not malignant. When seeded at low density, the cells grow as single cells. |
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| Propagation: | ATCC complete growth medium: The base medium for this cell line (MEBM) along with the additives can be obtained from Lonza/Clonetics Corporation as a kit: MEGM, Kit Catalog No. CC-3150. ATCC does not use the GA-1000 (gentamycin-amphotericin B mix) provided with kit. To make the complete growth medium, you will need to add the following components to the kit (sold separately):
Temperature: 37.0°C |
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| Subculturing: | Protocol:
Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:6 is recommended Medium Renewal: Every 2 to 3 days |
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| Preservation: | Culture medium, 75%; fetal bovine serum, 15%; glycerol, 10% | ||
| References: | 21894: . Transformation of human epithelial cells. Boca Raton, FL: CRC Press; 1992. 21926: Stampfer MR. Continuous human cell lines and method of making same. US Patent 4,808,532 dated Feb 28 1989 22413: Walen KH, Stampfer MR. Chromosome analyses of human mammary epithelial cells at stages of chemical-induced transformation progression to immortality. Cancer Genet. Cytogenet. 37: 249-261, 1989. PubMed: 2702624 23289: Stampfer MR, Bartley JC. Induction of transformation and continuous cell lines from normal human mammary epithelial cells after exposure to benzo[a]pyrene. Proc. Natl. Acad. Sci. USA 82: 2394-2398, 1985. PubMed: 3857588 |
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