- 详细信息
- 询价记录
- 文献和实验
- 技术资料
- 库存:
大量
- 物种来源:
小鼠
- 是否是肿瘤细胞:
0
- 免疫类型:
IgG2a
- 细胞形态:
淋巴样
- 运输方式:
冻存运输
- ATCC Number:
HB-241™
- 生长状态:
悬浮生长
| Designations: | 162-21.2 | ||
| Depositors: | LA Herzenberg | ||
| Isotype: | IgG2a | ||
| Biosafety Level: | 1 | ||
| Shipped: | frozen | ||
| Medium & Serum: | See Propagation | ||
| Growth Properties: | suspension | ||
| Organism: | Mus musculus (B cell); Mus musculus (myeloma) deposited as mouse (B cell); mouse (myeloma) | ||
| Morphology: | lymphoblast |
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| Source: | Cell Type: hybridoma: B lymphocyte; | ||
| Cellular Products: | immunoglobulin; monoclonal antibody; against Trop-1 antigen | ||
| Permits/Forms: | In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location. | ||
| Comments: | Animals were immunized with the human choriocarcinoma cell line BeWo. Spleen cells were fused with NS-1 myeloma cells. The antibody reacts with an antigen (Trop-1) present on trophoblast cells and on human choriocarcinomas. The antigen has also been detected on 4 of 4 human choriocarcinoma cell lines tested, on a fibrosarcoma cell line (HT1080C), on an erythroleukemia cell line (K562) and on the majority of human malignant epithelial cells and fetal epithelial precursors. The gene encoding the trop-1 antigen has been cloned. No staining was detected on peripheral blood cells. |
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| Propagation: | ATCC complete growth medium: The base medium for this cell line is ATCC-formulated RPMI-1640 Medium, Catalog No. 30-2001. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%. |
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| Subculturing: | Medium Renewal: Every 2 to 3 days Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 2 X 10 exp5 cells/ml and maintain between 1 X 10 exp5 and 1 X 10 exp6 cells/ml. |
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| References: | 1309: Lipinski M, et al. Human trophoblast cell-surface antigens defined by monoclonal antibodies. Proc. Natl. Acad. Sci. USA 78: 5147-5150, 1981. PubMed: 7029529 22152: Strnad J, et al. Molecular cloning and characterization of a human adenocarcinoma/epithelial cell surface antigen complementary DNA. Cancer Res. 49: 314-317, 1989. PubMed: 2463074 22157: Klein CE, et al. Expression of 38-kD cell-surface glycoprotein in transformed human keratinocyte cell lines, basal cell carcinomas, and epithelial germs. J. Invest. Dermatol. 95: 74-82, 1990. PubMed: 2366004 |
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文献和实验Crystallization of Kinesin Family Motor Proteins
.67 Åc=74.13 Å1.9 ÅMolecular replacement rK379PDB: 3KINP212121a=72.2 Åb=91.9 Åc=141.7 Å3.0 ÅMultiple isomorphous replacement Kar3 382-729PDB: 3KARP21a=44.1 Åb=81.2 Åc=48.3 Åß=105.8°2.3 ÅMolecular replacement and phases of three heavy atom derivatives
XBB.1.5 毒株的传播力为何这么强?北大曹云龙团队最新研究揭示相关机制
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P=0.05 (双侧) r r(较大均方的自由度) n 2 2 3 4 5 6 7 8 9 10 12 15 20 30 60 00 1 799 364 899 922 937 948 957 963 969 977 985 993 1001 1010 1018 1 2 39.0 39.2 39.2 39.3 39.3 39.3 39.4 39.4 39.4 39.4 39.4 39.4 39.5 39.5 39.5 2 3 10.0 15.4 15
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