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- 详细信息
- 询价记录
- 文献和实验
- 技术资料
- 运输方式：
冻存运输
- 物种来源：
人
- 是否是肿瘤细胞：
0
- 细胞类型：
其他细胞类型
- 组织来源：
duct
- 年限：
54 years adult
- 相关疾病：
导管癌
- ATCC Number：
CRL-2865™
- 细胞形态：
上皮样
- 库存：
大量
- 器官来源：
乳房
- 生长状态：
贴壁生长
| Designations: | T47D-KBluc | ||
| Depositors: | VS Wilson | ||
| Biosafety Level: | 1 | ||
| Shipped: | frozen | ||
| Medium & Serum: | See Propagation | ||
| Growth Properties: | adherent | ||
| Organism: | Homo sapiens | ||
| Morphology: | epithelial |
||
| Source: | Organ: mammary gland; breast Tissue: duct Disease: ductal carcinoma Derived from metastatic site: pleural effusion Cell Type: epithelial transfected with reporter plasmid |
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| Permits/Forms: | In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location. | ||
| Isolation: | Isolation date: January 4, 2000 | ||
| Applications: | They can be used to screen chemicals for estrogenic or anti-estrogenic activity. | ||
| Reverse Transcript: | N | ||
| DNA Profile (STR): | Amelogenin: X CSF1PO: 11,13 D13S317: 12 D16S539: 10 D5S818: 12 D7S820: 11 THO1: 6 TPOX: 11 vWA: 14 |
||
| Age: | 54 years adult | ||
| Gender: | female | ||
| HeLa Markers: | N | ||
| Comments: | The parent cell line was transfected with pGL2.TATA.Inr.luc.neo which contains three estrogen responsive elements upstream of a luc reporter gene. The cells were selected for responsiveness to 17beta-estradiol. They can be used to screen chemicals for estrogenic or anti-estrogenic activity. | ||
| Propagation: | ATCC complete growth medium: The base medium for this cell line is ATCC-formulated RPMI-1640 Medium, Catalog No. 30-2001. To make the complete growth medium, add the following components to the base medium: 0.2 Units/ml bovine insulin; fetal bovine serum to a final concentration of 10%. Atmosphere: air, 95%; carbon dioxide (CO2), 5% Temperature: 37.0°C Duration: The depositor states that supplementing the growth medium with insulin is optional. |
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| Subculturing: | Protocol:
Subcultivation Ratio: 1:2 to 1:3 is recommended Medium Renewal: every 2 to 3 days |
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| Preservation: | Freeze medium: culture medium, 95%; DMSO, 5% Storage temperature: liquid nitrogen vapor phase |
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| Doubling Time: | 36 | ||
| Related Products: | Recommended medium (without the additional supplements or serum described under ATCC Medium):ATCC 30-2001 recommended serum:ATCC 30-2020 parental cell line:ATCC HTB-133 0.25% (w/v) Trypsin - 0.53 mM EDTA in Hank' BSS (w/o Ca++, Mg++):ATCC 30-2101 Hanks' Balanced Salt Solution (w/o Ca++, Mg++, phenol red):ATCC 30-2213 |
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| References: | 89919: Wilson VS, et al. Development and characterization of a cell line that stably expresses an estrogen-responsive luciferase reporter for the detection of estrogen receptor agonist and antagonists. Toxicol. Sci. 81: 69-77, 2004. PubMed: 15166400 | ||
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文献和实验Use of three-dimensional collagen gels to study mechanotransduction in t47d breast epithelial cells
of in vitro models to understand how both biophysical and biochemical signals regulate complex cellular behaviors. T47D breast epithelial cells will differentiate into duct-like tubules when cultured in a floating three-dimensional (3D) collagen gel
HBC裸鼠移植模型的建立包括活检取材的HBC标本和细胞株，后者应用较广泛。本贴主要讨论后者，算是抛砖了！1、人乳腺癌细胞株的选择目前人乳腺癌细胞建株的癌细胞较多，本人曾接触过细胞株总结如下：ER（+）：MCF-7（来源：乳腺腺癌），ZR-75-30（乳腺导管癌），T47D（乳腺导管癌），ZR-75（乳腺腺癌）。ER（-）：MDA-MB-435s（乳腺导管癌），MDA-MB-435（乳腺导管癌），SK-BR-3 （乳腺腺癌），MDA-MB-231（乳腺腺癌），MDA-MB-453（乳腺
Nature 深度剖析：管坤良与 Alj 组研究成果为何截然相反，Hippo 通路调控乳腺癌的两面性
ERα，在癌细胞系 T47D 中敲降 LATS1 不影响 ESR1 mRNA 水平（Extended Fig 8b）。考虑到方法的不同，作者采用了上文作者使用的 shRNA 敲降 LATS1/2，但发现敲降 LATS1/2 既不影响 ERα 水平，也不活化 YAP/TAZ。作者猜测，可能是残余的 LATS1/2 的量仍足以抑制 YAP/TAZ 活化。此外，在其它 ER + 乳腺癌细胞系，及小鼠的输卵管、乳腺和子宫内膜类器官（organoids）模型中，敲除 LATS1/2 均可抑制 ESR
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