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- ATCC Number：
HB-32™
- 库存：
大量
- 免疫类型：
IgG2a; kappa light chain
- 细胞形态：
淋巴样
- 运输方式：
冻存运输
- 物种来源：
小鼠
- 是否是肿瘤细胞：
0
- 生长状态：
悬浮生长
| Designations: | 14-4-4S | ||
| Depositors: | DH Sachs, K Ozato | ||
| Isotype: | IgG2a; kappa light chain | ||
| Biosafety Level: | 1 | ||
| Shipped: | frozen | ||
| Medium & Serum: | See Propagation | ||
| Growth Properties: | suspension | ||
| Organism: | Mus musculus (B cell); Mus musculus (myeloma) deposited as mouse (B cell); mouse (myeloma) | ||
| Morphology: | lymphoblast |
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| Source: | Cell Type: hybridoma: B lymphocyte; | ||
| Cellular Products: | immunoglobulin; monoclonal antibody; against I-E k; cytotoxic | ||
| Permits/Forms: | In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location. | ||
| Comments: | Animals were immunized with spleen cells from C3H mice. Spleen cells were fused with Sp2/0-Ag14 myeloma cells. The antibody is highly cross-reactive and reacts with H-2k, H-2d, H-2p and H-2r. The specificity of the antibody corresponds to the Ia.7 serologic specificity. Tested and found negative for ectromelia virus (mousepox). |
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| Propagation: | ATCC complete growth medium: The base medium for this cell line is ATCC Hybri-Care Medium, Catalog No. 46-X. Hybri-Care Medium is supplied as a powder and should be reconstituted in 1 L cell-culture-grade water. To make the complete growth medium, add the following components to the base medium: Temperature: 37.0°C Atmosphere: air, 95%; carbon dioxide (CO2), 5% |
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| Subculturing: | Medium Renewal: Every 2 to 3 days Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 2 X 10 exp5 cells/ml and maintain between 1 X 10 exp5 and 1 X 10 exp6 cells/ml. |
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| Preservation: | Freeze medium: complete growth medium, 95%; DMSO, 5% | ||
| Related Products: | Recommended medium (without the additional supplements or serum described under ATCC Medium):ATCC 46-X | ||
| References: | 905: Ozato K, et al. Hybridoma cell lines secreting monoclonal antibodies to mouse H-2 and Ia antigens. J. Immunol. 124: 533-540, 1980. PubMed: 7188699 | ||
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14-4-4S
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