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- 2025年11月04日
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- 文献和实验
- 技术资料
- 库存：
大量
- 运输方式：
冻存运输
- 生长状态：
混合型生长
- 物种来源：
大鼠
- 是否是肿瘤细胞：
0
- ATCC Number：
HB-58™
- 免疫类型：
IgG1
- 细胞形态：
淋巴样
| Designations: | 187.1 | ||
| Depositors: | MD Scharff | ||
| Isotype: | IgG1 | ||
| Biosafety Level: | 1 | ||
| Shipped: | frozen | ||
| Medium & Serum: | See Propagation | ||
| Growth Properties: | mixed suspension-adherent | ||
| Organism: | Rattus norvegicus (B cell); Mus musculus (myeloma) deposited as rat (B cell); mouse (myeloma) | ||
| Morphology: | lymphoblast |
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| Source: | Cell Type: hybridoma: B lymphocyte; | ||
| Cellular Products: | immunoglobulin; monoclonal antibody; against mouse kappa light chain | ||
| Permits/Forms: | In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location. | ||
| Comments: | Animals were immunized with MPC-11 immunoglobulin. Spleen cells were fused with P3X63Ag8.653 myeloma cells. Tested and found negative for ectromelia virus (mousepox). |
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| Propagation: | ATCC complete growth medium: The base medium for this cell line is ATCC Hybri-Care Medium, Catalog No. 46-X. Hybri-Care Medium is supplied as a powder and should be reconstituted in 1 L cell-culture-grade water. To make the complete growth medium, add the following components to the base medium: |
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| Subculturing: | Medium Renewal: Every 2 to 3 days Subculture by scraping attached cells and transferring with floating cells to a new flask. Start cultures at 2 X 10 (5) cells/ml and maintain between 1 X 10 (5) and 1 X 10 (6) cells/ml. |
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| Preservation: | Freeze medium: complete growth medium, 95%; DMSO, 5% | ||
| Related Products: | Recommended medium (without the additional supplements or serum described under ATCC Medium):ATCC 46-X | ||
| References: | 32236: Brumeanu TD, et al. Immunogenicity of a contiguous T-B synthetic epitope of the A/PR/8/34 influenza virus. J. Virol. 71: 5473-5480, 1997. PubMed: 9188620 57995: Yelton DE, et al. Use of monoclonal anti-mouse immunoglobulin to detect mouse antibodies. Hybridoma 1: 5-11, 1981. PubMed: 6821395 |
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