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- 相关疾病：
正常
- 库存：
大量
- ATCC Number：
CRL-10685™
- 年限：
embryo
- 细胞形态：
成纤维样
- 品系：
NIH/Swiss
- 生长状态：
贴壁生长
- 运输方式：
冻存运输
- 物种来源：
小鼠
- 是否是肿瘤细胞：
0
| Designations: | PG13/LN c8 | ||
| Depositors: | Fred Hutchinson Cancer Res. Cntr. | ||
| Biosafety Level: | 2 [CELLS CONTAIN RETROVIRUS ] | ||
| Shipped: | frozen | ||
| Medium & Serum: | See Propagation | ||
| Growth Properties: | adherent | ||
| Organism: | Mus musculus | ||
| Morphology: | fibroblast |
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| Source: | Disease: normal Strain: NIH/Swiss |
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| Cellular Products: | a Gibbon ape Leukemia Virus (GaLV) based neomycin resistance transducing vector | ||
| Permits/Forms: | In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location. | ||
| Reverse Transcript: | positive | ||
| Age: | embryo | ||
| Comments: | PG13/LN c8 cells produce a retroviral vector (LN) that transduces the neo gene and can infect cells from many mammalian species (other than mice). The line was derived from PG13 (see ATCC CRL-10686 ). Although virus production has not been observed, there is a possibility that the cells will produce a virus similar to GaLV (a moderate risk oncogenic virus) and Biosafety Level 2 or higher precautions should be taken when using this cell line. |
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| Propagation: | ATCC complete growth medium: The base medium for this cell line is ATCC-formulated Dulbecco's Modified Eagle's Medium, Catalog No. 30-2002. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%. Temperature: 37.0°C |
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| Subculturing: | Subcultivation Ratio: A subcultivation ratio of 1:4 is recommended Medium Renewal: Every 2 to 3 days Remove medium, add fresh 0.25% Trypsin, 0.02% EDTA solution and allow the flask to sit at room temperature (or 37C) until the cells detach (2 to 3 minutes). Add fresh medium, aspirate and dispense into new flasks. |
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| References: | 1818: Miller AD, et al. Construction and properties of retrovirus packaging cells based on gibbon ape leukemia virus. J. Virol. 65: 2220-2224, 1991. PubMed: 1850008 4473: Miller AD, Rosman GJ. Improved retroviral vectors for gene transfer and expression. BioTechniques 7: 980-990, 1989. PubMed: 2631796 |
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