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- 细胞形态：
成纤维样
- 器官来源：
皮肤
- 物种来源：
人
- 是否是肿瘤细胞：
0
- 相关疾病：
着色性干皮病
- ATCC Number：
CRL-1172™
- 生长状态：
贴壁生长
- 库存：
大量
- 运输方式：
冻存运输
- 年限：
45 years
| Designations: | Win Mec | ||
| Biosafety Level: | 1 | ||
| Shipped: | frozen | ||
| Medium & Serum: | See Propagation | ||
| Growth Properties: | adherent | ||
| Organism: | Homo sapiens | ||
| Morphology: | fibroblast |
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| Source: | Organ: skin Disease: xeroderma pigmentosum, presumed heterozygote |
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| Permits/Forms: | In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location. | ||
| Age: | 45 years | ||
| Gender: | female | ||
| Comments: | The tissue donor was a presumed heterozygote for xeroderma pigmentosum, and the mother of Wo Mec (ATCC CRL-1162). | ||
| Propagation: | ATCC complete growth medium: The base medium for this cell line is ATCC-formulated Dulbecco's Modified Eagle's Medium, Catalog No. 30-2002. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%. |
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| Subculturing: | Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:3 is recommended Medium Renewal: 2 to 3 times per week Remove spent medium, add fresh 0.25% trypsin for 1 to 2 minutes, remove trypsin and let the culture sit at room temperature for 5 to 10 minutes. Add fresh medium, aspirate and dispense into new flasks. Subculture every 6 to 8 days. |
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