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- 技术资料
- ATCC Number：
CCL-75.1™
- 库存：
大量
- 物种来源：
人
- 是否是肿瘤细胞：
0
- 细胞类型：
其他细胞类型
- 运输方式：
冻存运输
- 细胞形态：
上皮样
- 器官来源：
肺
- 年限：
3 months gestation
- 生长状态：
贴壁生长
| Designations: | WI-38 VA-13 subline 2RA | ||
| Depositors: | AJ Girardi | ||
| Biosafety Level: | 2 [Cells contain polyomavirus DNA sequences ] | ||
| Shipped: | frozen | ||
| Medium & Serum: | See Propagation | ||
| Growth Properties: | adherent | ||
| Organism: | Homo sapiens | ||
| Morphology: | epithelial |
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| Source: | Organ: lung Cell Type: fibroblastSV40 transformed |
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| Permits/Forms: | In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location. | ||
| Isolation: | Isolation date: March, 1964 | ||
| Applications: | transfection host | ||
| Virus Susceptibility: | Human poliovirus 2 Herpes simplex virus Vesicular stomatitis virus |
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| DNA Profile (STR): | Amelogenin: X CSF1PO: 10,12 D13S317: 11 D16S539: 11,12 D5S818: 10 D7S820: 9,11 THO1: 9.3 TPOX: 8 vWA: 19,20 |
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| Cytogenetic Analysis: | modal number = 76; range = 45 to 89. Karyotype unstable within hyperdiploid stemline range 73-78. Ninety percent of the cells examined have 2 or 3 large chromosomes with subterminal centromeres and 1-6 microchromosomes. Secondary constrictions were observed in 59% of the cells, 21% had breaks. |
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| Isoenzymes: | G6PD, B | ||
| Age: | 3 months gestation | ||
| Gender: | female | ||
| Ethnicity: | Caucasian | ||
| Propagation: | ATCC complete growth medium: The base medium for this cell line is ATCC-formulated Eagle's Minimum Essential Medium, Catalog No. 30-2003. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%. Temperature: 37.0°C |
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| Subculturing: | Protocol:
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| Preservation: | Freeze medium: Complete growth medium supplemented with 5% (v/v) DMSO Storage temperature: liquid nitrogen vapor phase |
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| Related Products: | Recommended medium (without the additional supplements or serum described under ATCC Medium):ATCC 30-2003 recommended serum:ATCC 30-2020 parental cell line:ATCC CCL-75 |
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| References: | 23133: Girardi AJ, et al. SV40 transformation of human diploid cells. A parallel study of viral and karyologic parameters. Ann. Med. Exp. Biol. Fenn. 44: 242-254, 1966. PubMed: 4290661 26230: Jensen F, et al. Autologous and homologous implantation of human cells transformed in vitro by simian virus 40. J. Natl. Cancer Inst. 32: 917-937, 1964. 32701: Wang S, Vos JM. A hybrid herpesvirus infectious vector based on epstein-barr virus and herpes simplex virus type 1 for gene transfer into human cells in vitro and in vivo. J. Virol. 70: 8422-8430, 1996. PubMed: 8970963 |
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