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- 细胞形态：
淋巴样
- 相关疾病：
其他疾病
- ATCC Number：
CRL-1823™
- 库存：
大量
- 运输方式：
冻存运输
- 生长状态：
悬浮生长
- 物种来源：
人
- 是否是肿瘤细胞：
0
- 器官来源：
淋巴结
| Designations: | K6H6/B5 | ||
| Depositors: | R Levy | ||
| Biosafety Level: | 1 | ||
| Shipped: | frozen | ||
| Medium & Serum: | See Propagation | ||
| Growth Properties: | suspension | ||
| Organism: | human (B cell lymphoma); mouse (myeloma) | ||
| Morphology: | lymphoblast |
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| Source: | Organ: lymph node Disease: nodular lymphoma Cell Type: hybridoma: B lymphocyte; somatic cell hybrid |
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| Permits/Forms: | In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location. | ||
| Applications: | The line was produced from a fusion of P3/NSI/1-Ag4-1 cells with malignant cells from a human nodular lymphoma. The cells are deficient in hypoxanthine phosphoribosyltransferase (HPRT) and are excellent fusion partners for human B cells. Originally the cells secreted human IgM with a lambda light chain; however, with continuous passage they spontaneously lost the ability to secrete immunoglobulin. Tested and found negative for ectromelia virus (mousepox). |
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| Comments: | The line was produced from a fusion of P3/NSI/1-Ag4-1 cells with malignant cells from a human nodular lymphoma. Originally the cells secreted human IgM with a lambda light chain; however, with continuous passage they spontaneously lost the ability to secrete immunoglobulin. The cells are deficient in hypoxanthine phosphoribosyltransferase (HPRT) and are excellent fusion partners for human B cells. Tested and found negative for ectromelia virus (mousepox). |
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| Propagation: | ATCC complete growth medium: The base medium for this cell line is ATCC-formulated RPMI-1640 Medium, Catalog No. 30-2001. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%. Temperature: 37.0°C Atmosphere: air, 95%; carbon dioxide (CO2 ), 5% |
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| Subculturing: | Protocol: Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 2 X 10(5) cells/ml and maintain between 1 X 10(5) and 1 X 10(6) cells/ml. Medium Renewal: Every 2 to 3 days |
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| Preservation: | Freeze medium: complete growth medium, 95%; DMSO, 5% Storage temperature: liquid nitrogen vapor phase |
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| Related Products: | Recommended medium (without the additional serum described under ATCC Medium): ATCC 30-2001 Recommended serum: ATCC 30-2020 Cell culture tested DMSO: ATCC 4-X Erythrosin B vital stain solution: ATCC 30-2404 |
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| References: | 1625: Carroll WL, et al. Mouse X human heterohybridomas as fusion partners with human B cell tumors. J. Immunol. Methods 89: 61-72, 1986. PubMed: 3084658 | ||
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文献和实验Pollen Development in Anthers of Arabidopsis
in Petri dishes on a layer of sterilized superfine grade Perlite pre-wetted with Gamborg's B5 Medium (see Growing Arabidopsis protocol). 2. Incubate in a growth chamber at 19-23°C under cool white fluorescent light (1500 to 2200 lux).
扫描仪中，都采用机械式的二维X，Y线性扫描技术实现，即X，Y方向都采用直线驱动器和直线导轨实现往复运动。此类装置，由于驱动系统的频率限制，驱动器的扫描惯性大，使得扫描效率低，分析时间相当长；并且往复行程长，对直线导轨的精度要求相当高。二、光机结合的二维扫描系统为同样实现生物芯片的二维扫描，我们的实验装置设计如图2，采用了振镜和大数值孔径的远心f-è物镜相结合实现X方向扫描，Y方向的运动仍采用直线驱动器和直线导轨实现。 系统中，对于f-è物镜，满足x＝2fè（è为振镜的摆动角度，f为物镜焦距）的线性
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