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- 免疫类型：
IgG2b
- 细胞形态：
淋巴样
- 运输方式：
冻存运输
- ATCC Number：
HB-9173™
- 生长状态：
悬浮生长
- 库存：
大量
- 物种来源：
小鼠
- 是否是肿瘤细胞：
0
| Designations: | XMMCO-791 | ||
| Depositors: | Xoma Corporation | ||
| Isotype: | IgG2b | ||
| Biosafety Level: | 1 | ||
| Shipped: | frozen | ||
| Medium & Serum: | See Propagation | ||
| Growth Properties: | suspension | ||
| Organism: | Mus musculus (B cell); Mus musculus (myeloma) deposited as mouse (B cell); mouse (myeloma) | ||
| Morphology: | lymphoblast |
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| Source: | Cell Type: hybridoma: B lymphocyte; | ||
| Cellular Products: | immunoglobulin; monoclonal antibody; against colorectal carcinoma associated tumor antigen | ||
| Permits/Forms: | In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location. | ||
| Comments: | Animals were immunized with the 791T osteogenic sarcoma cell line (see ATCC CRL-7798). Spleen cells were fused with P3-NSI/1-Ag4-1 myeloma cells. The antibody reacts with a tumor associated antigen present in human colorectal carcinoma. |
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| Propagation: | ATCC complete growth medium: The base medium for this cell line is ATCC-formulated Dulbecco's Modified Eagle's Medium, Catalog No. 30-2002. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%. Temperature: 37.0°C |
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| Subculturing: | Medium Renewal: Every 2 to 3 days Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 2 X 10 exp5 cells/ml and maintain between 1 X 10 exp5 and 1 X 10 exp6 cells/ml. |
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| Preservation: | culture medium 95%; DMSO, 5% | ||
| Related Products: | Recommended medium (without the additional supplements or serum described under ATCC Medium):ATCC 30-2002 recommended serum:ATCC 30-2020 |
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| References: | 3918: Baldwin RW. Radioimmuno detection of human cancers using anti-tumor monoclonal antibody. US Patent 4,708,862 dated Nov 24 1987 | ||
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文献和实验Detection of Circulating Tumor Cells by Photoacoustic Flowmetry
Detection of circulating tumor cells (CTCs) in human blood and lymph systems has the potential to aid clinical decision making in the treatment of cancer (Cristofanilli et al. New Engl J Med 351:781–791, 2004; Check Cap Today 19:1.76–1.86
柳叶刀子刊报道离奇病例，损失四分之一大脑皮质的他竟与常人无异！
Brains. Neuron, 2017. 95(4): p. 791-807.e7.4.Newbold, D.J., et al., Plasticity and Spontaneous Activity Pulses in Disused Human Brain Circuits. Neuron, 2020. 107(3): p. 580-589.e6.
Subcloning: restriction enzyme digests, ligation
diphosphates and triphosphates and selectivity identical to that of the wild-type receptor. Biochemical Pharmacology 59, 791-800). You will isolate a 1.4kb PstI-BamHI DNA fragment (insert) containing the amplified P2X2 sequence (that you generated using
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