- 询价
- 2025年12月14日
企业认证
- 详细信息
- 文献和实验
- 技术资料
- 物种来源：
人
- 是否是肿瘤细胞：
0
- 组织来源：
uroepithelium
- 库存：
大量
- 细胞形态：
上皮样
- 年限：
11 years
- 运输方式：
冻存运输
- ATCC Number：
CRL-9519™
- 器官来源：
输尿管
- 生长状态：
贴壁生长
| Designations: | MC-SV-HUC T-2 | ||
| Depositors: | Wisconsin Alumni Res. Fndn. | ||
| Biosafety Level: | 2 [Cells contain polyomavirus DNA sequences ] | ||
| Shipped: | frozen | ||
| Medium & Serum: | See Propagation | ||
| Growth Properties: | adherent | ||
| Organism: | Homo sapiens | ||
| Morphology: | epithelial |
||
| Source: | Organ: ureter Tissue: uroepithelium |
||
| Cellular Products: | uroepithelial keratins | ||
| Permits/Forms: | In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location. | ||
| Tumorigenic: | Yes | ||
| Age: | 11 years | ||
| Gender: | male | ||
| Comments: | The cells were initially transformed with SV40 virus, and later treated with 3-methylcholanthrene (MCA). While the parental line (SV-HUC-1, see ATCC CRL-9520 ) was not tumorigenic and had a balanced chromosome composition, the MCA treated line became tumorigenic and aneuploid. The line has been repeatedly tested for production of infectious SV40 using an African Green Monkey kidney cell plaque assay, and has always tested negative. Stress (such as chemical exposure) could possibly activate the virus. |
||
| Propagation: | ATCC complete growth medium: Ham's F12 medium, 90%; fetal bovine serum, 10% Temperature: 37.0°C |
||
| Subculturing: | Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:5 is recommended Medium Renewal: Every 2 to 3 days Remove medium, and rinse with 0.25% trypsin, 0.03% EDTA solution. Remove the solution and add an additional 1 to 2 ml of trypsin-EDTA solution. Allow the flask to sit at room temperature (or at 37C) until the cells detach. Add fresh culture medium, aspirate and dispense into new culture flasks. |
||
| Preservation: | culture medium 95%; DMSO, 5% | ||
| Related Products: | recommended serum:ATCC 30-2020 | ||
| References: | 3992: Reznikoff CA, Christian BJ. Human uroepithelial cell. US Patent 4,980,290 dated Dec 25 1990 | ||
风险提示：丁香通仅作为第三方平台，为商家信息发布提供平台空间。用户咨询产品时请注意保护个人信息及财产安全，合理判断，谨慎选购商品，商家和用户对交易行为负责。对于医疗器械类产品，请先查证核实企业经营资质和医疗器械产品注册证情况。
文献和实验细胞治疗技术正扮演着越来越重要的角色。其中，CAR-T 细胞治疗、间充质干细胞（MSC）治疗以及 T 细胞治疗是三种备受关注的细胞治疗方法。 这些治疗方法通过采集患者体内的特定细胞，经过体外激活、修饰、扩增等步骤，再将这些具有特定功能的细胞移植回患者体内，以达到治疗疾病的目的。为了更好地理解和应用这些治疗技术，下面我们将整合并详细叙述这三种细胞治疗的流程图内容，为研究人员和临床医生提供一个清晰的操作指南。 CAR-T 细胞治疗流程 间充质干细胞治疗流程 T 细胞治疗流程
网络 附表3 t值表 n’ P(2): P(1): 0.50 0.25 0.20 0.10 0.10 0.05 0.05 0.025 0.02 0.01 0.01 0.005 0.005 0.0025 0.002 0.001 0.001
一光年 最近要做生殖的细胞方面的实验，前期一直用在垂体细胞原代培养，但是一直困扰没有促性腺激素细胞系，但是在网络中搜索，发现老外的文章中经常提到αT3-1，LβT2 或LβT4细胞系，在ATCC等知名的细胞库也未发现这些细胞系，不知道各位高手指点在哪里可以找到这几种细胞系？不甚感激！！ veimojie 我一直也在做这方面的研究 什么地方能有啊 ？ banana_smile 我也想做这方
技术资料暂无技术资料 索取技术资料
