- 询价
- 2026年08月18日
企业认证
万千商家帮你免费找货
0 人在求购买到急需产品
相关阅读
阿拉丁试剂，充足现货，稳定批间差Cytoscape 教程来了！快速实现顶刊同款通路网络图五大应用案例：Mustang Q 膜层析应用全解析1 个小工具，一次性搞定流程图、质粒图谱和信号通路图- 详细信息
- 文献和实验
- 技术资料
- 运输方式：
冻存运输
- 相关疾病：
其他疾病
- ATCC Number：
CRL-1501™
- 物种来源：
人
- 是否是肿瘤细胞：
0
- 年限：
1 week
- 细胞类型：
成纤维细胞
- 细胞形态：
成纤维样
- 库存：
大量
- 生长状态：
贴壁生长
- 器官来源：
皮肤
| Designations: | CCD-39Sk | ||
| Biosafety Level: | 1 | ||
| Shipped: | frozen | ||
| Medium & Serum: | See Propagation | ||
| Growth Properties: | adherent | ||
| Organism: | Homo sapiens | ||
| Morphology: | fibroblast |
||
| Source: | Organ: skin Disease: hyaline membrane disease Cell Type: fibroblast |
||
| Permits/Forms: | In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location. | ||
| DNA Profile (STR): | Amelogenin: X CSF1PO: 10,12 D13S317: 11 D16S539: 11,13 D5S818: 11,12 D7S820: 9,12 THO1: 6,9.3 TPOX: 8,11 vWA: 14,17 |
||
| Age: | 1 week | ||
| Gender: | female | ||
| Comments: | The line was derived from normal skin of a child that died of hyaline membrane disease. | ||
| Propagation: | ATCC complete growth medium: The base medium for this cell line is ATCC-formulated Eagle's Minimum Essential Medium, Catalog No. 30-2003. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%. Atmosphere: air, 95%; carbon dioxide (CO2), 5% Temperature: 37.0°C |
||
| Subculturing: | Protocol:
Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:4 is recommended Medium Renewal: 2 to 3 times per week |
||
| Preservation: | Freeze medium: Complete growth medium supplemented with 5% (v/v) DMSO Storage temperature: liquid nitrogen vapor phase |
||
| Related Products: | Recommended medium (without the additional supplements or serum described under ATCC Medium):ATCC 30-2003 recommended serum:ATCC 30-2020 |
||
风险提示：丁香通仅作为第三方平台，为商家信息发布提供平台空间。用户咨询产品时请注意保护个人信息及财产安全，合理判断，谨慎选购商品，商家和用户对交易行为负责。对于医疗器械类产品，请先查证核实企业经营资质和医疗器械产品注册证情况。
文献和实验) Hs 257.T CRL-7400 Homo sapiens (human) Hs 675.T CRL-7435 Homo sapiens (human) Hs 698.T HTB-37 Homo sapiens (human) Caco-2 HTB-38 Homo sapiens (human) HT-29 HTB-39 Homo sapiens (human) SK-CO-1 HTB-78 Homo sapiens (human) SW 626
CACTG ACAGG CAATA CCGTC CAAGG 5.3 kb 18S rRNA 大豆 有意义链CTTTC GATGG TAGGA TAGTG GCCT反义链CAATG ATCCT TCCGC AGGTT CACCT AC 1.5 kb *引物不会扩增假基因 PCR引物序列 基 因 来源 引物 序 列 产物大小（kb） HIV gag region 病毒 SK 38ATTAAT CACTA TCCAG TAGGA GAAAT SK 39TTTGG TCCTG
+~40+。qPCR结果CT值通常在30以上为默认不表达，但外泌体比较特殊，可适当放宽标准。 17、外泌体q PCR实验如何使用内参？ 答：外泌体没有公认的内参，常规内参基因在外泌体中表达量甚至可能比目标基因还低。外参是在没有合适内参的情况下的替代物。microRNA检测外参是化学合成的线虫短单片段链RNA (cel-miR-39-3p)模仿，经检测在、小鼠、吸附中均无同源片段，最大限度地降低了对名义实验造成的干扰，适用于内参表达人类不稳定的外泌体或暂未发现合适内参的其他样品，对目的
技术资料暂无技术资料 索取技术资料
