CCD-986Sk产品图

CCD-986Sk

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  • 询价
  • 2026年08月18日
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    • 详细信息
    • 文献和实验
    • 技术资料
    • 库存

      大量

    • 生长状态

      贴壁生长

    • 物种来源

    • 是否是肿瘤细胞

      0

    • 运输方式

      冻存运输

    • 年限

      22 years

    • 细胞类型

      成纤维细胞

    • 细胞形态

      成纤维样

    • 相关疾病

      正常

    • 器官来源

      皮肤

    • ATCC Number

      CRL-1947™

    Designations: CCD-986Sk
    Biosafety Level: 1
    Shipped: frozen
    Medium & Serum: See Propagation
    Growth Properties: adherent
    Organism: Homo sapiens
    Morphology: fibroblast

    Source: Organ: skin
    Disease: normal
    Cell Type: fibroblast
    Permits/Forms: In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.
    DNA Profile (STR): Amelogenin: X
    CSF1PO: 11,13
    D13S317: 9,12
    D16S539: 8
    D5S818: 11,13
    D7S820: 10,11
    THO1: 7,9.3
    TPOX: 8,11
    vWA: 14,17
    Age: 22 years
    Gender: female
    Ethnicity: Black
    Comments: The line was established from skin taken from normal breast tissue removed during breast reduction mammoplasty.
    The cells have been observed to senesce at about 36 population doublings.
    Propagation: ATCC complete growth medium: The base medium for this cell line is ATCC-formulated Iscove's Modified Dulbecco's Medium, Catalog No. 30-2005. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
    Temperature: 37.0°C
    Atmosphere: air, 95%; carbon dioxide (CO2), 5%
    Subculturing: Protocol:
    1. Remove and discard culture medium.
    2. Briefly rinse the cell layer with 0.25% (w/v) Trypsin- 0.53 mM EDTA solution to remove all traces of serum which contains trypsin inhibitor.
    3. Add 2.0 to 3.0 ml of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).
      Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37�C to facilitate dispersal.
    4. Add 6.0 to 8.0 ml of complete growth medium and aspirate cells by gently pipetting.
    5. Add appropriate aliquots of the cell suspension to new culture vessels.
    6. Incubate cultures at 37�C.

    Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:3 is recommended
    Medium Renewal: Every 2 to 3 days
    Preservation: Freeze medium: Complete growth medium 95%; DMSO, 5%
    Storage temperature: liquid nitrogen vapor phase
    Related Products: Recommended medium (without the additional supplements or serum described under ATCC Medium):ATCC 30-2005
    recommended serum:ATCC 30-2020

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    图标文献和实验
    相关实验
    • 【求助】关于结肠癌细胞系

      sapiens (human) LS 180 CL-188 Homo sapiens (human) LS 174T CRL-10624 Homo sapiens (human) CO 88BV59-1 CRL-11538 Homo sapiens (human) Co88BV59H21-2 CRL-11539 Homo sapiens (human) Co88BV59H21-2V67-66 CRL-1459 Homo sapiens (human) CCD-18

    • 中国科学院细胞库可供应细胞目录

      乳腺管癌细胞  400元 BxPC-3  TCHu 12  人  原位胰腺腺癌细胞  450元 C127  TCM26  小鼠  乳腺肿瘤细胞  400元 C6  TCR 1  大鼠  胶质瘤细胞  1000元 Caco-2  TCHU 66  人  结肠腺癌细胞  400元 Calu-3  TCHu 67  人  肺腺癌 (胸膜渗出液)  400元 CBRH-7919  TCR 2  大鼠  肝癌细胞  400元 CCD-1095Sk  GNHu15  人  乳腺浸润性导管癌旁皮肤

    • 细胞名称大汇总

      胚肺细胞 293 Cells, low passage  人胚肾细胞       293T         人胚肾细胞 AAV-293          胚肾细胞        hFOB 1.19      SV40 转染成骨细胞 CCD-1095Sk        皮肤细胞        Hs 578Bst      乳腺细胞 NK-92        人恶性非霍奇金淋巴瘤患者的自然杀伤细胞,   NK-92MI       人恶性非霍奇金淋巴瘤患者的自然

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