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- 文献和实验
- 技术资料
- 库存:
大量
- 生长状态:
贴壁生长
- 物种来源:
人
- 是否是肿瘤细胞:
0
- 运输方式:
冻存运输
- 年限:
22 years
- 细胞类型:
成纤维细胞
- 细胞形态:
成纤维样
- 相关疾病:
正常
- 器官来源:
皮肤
- ATCC Number:
CRL-1947™
| Designations: | CCD-986Sk | ||
| Biosafety Level: | 1 | ||
| Shipped: | frozen | ||
| Medium & Serum: | See Propagation | ||
| Growth Properties: | adherent | ||
| Organism: | Homo sapiens | ||
| Morphology: | fibroblast |
||
| Source: | Organ: skin Disease: normal Cell Type: fibroblast |
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| Permits/Forms: | In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location. | ||
| DNA Profile (STR): | Amelogenin: X CSF1PO: 11,13 D13S317: 9,12 D16S539: 8 D5S818: 11,13 D7S820: 10,11 THO1: 7,9.3 TPOX: 8,11 vWA: 14,17 |
||
| Age: | 22 years | ||
| Gender: | female | ||
| Ethnicity: | Black | ||
| Comments: | The line was established from skin taken from normal breast tissue removed during breast reduction mammoplasty. The cells have been observed to senesce at about 36 population doublings. |
||
| Propagation: | ATCC complete growth medium: The base medium for this cell line is ATCC-formulated Iscove's Modified Dulbecco's Medium, Catalog No. 30-2005. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%. Temperature: 37.0°C Atmosphere: air, 95%; carbon dioxide (CO2), 5% |
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| Subculturing: | Protocol:
Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:3 is recommended Medium Renewal: Every 2 to 3 days |
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| Preservation: | Freeze medium: Complete growth medium 95%; DMSO, 5% Storage temperature: liquid nitrogen vapor phase |
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| Related Products: | Recommended medium (without the additional supplements or serum described under ATCC Medium):ATCC 30-2005 recommended serum:ATCC 30-2020 |
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文献和实验sapiens (human) LS 180 CL-188 Homo sapiens (human) LS 174T CRL-10624 Homo sapiens (human) CO 88BV59-1 CRL-11538 Homo sapiens (human) Co88BV59H21-2 CRL-11539 Homo sapiens (human) Co88BV59H21-2V67-66 CRL-1459 Homo sapiens (human) CCD-18
乳腺管癌细胞 400元 BxPC-3 TCHu 12 人 原位胰腺腺癌细胞 450元 C127 TCM26 小鼠 乳腺肿瘤细胞 400元 C6 TCR 1 大鼠 胶质瘤细胞 1000元 Caco-2 TCHU 66 人 结肠腺癌细胞 400元 Calu-3 TCHu 67 人 肺腺癌 (胸膜渗出液) 400元 CBRH-7919 TCR 2 大鼠 肝癌细胞 400元 CCD-1095Sk GNHu15 人 乳腺浸润性导管癌旁皮肤
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