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- 详细信息
- 技术资料
- 细胞形态：
成纤维样
- 生长状态：
贴壁生长
- 运输方式：
冻存运输
- 库存：
大量
- 物种来源：
其他
- 是否是肿瘤细胞：
0
- 器官来源：
卵巢
- 细胞类型：
成纤维细胞
- ATCC Number：
CRL-2772™
- 年限：
juvenile
| Designations: | Channel Catfish Ovary [CCO] | ||
| Depositors: | L Hanson | ||
| Biosafety Level: | 1 | ||
| Shipped: | frozen | ||
| Medium & Serum: | See Propagation | ||
| Growth Properties: | adherent | ||
| Organism: | Ictalurus punctatus | ||
| Morphology: | fibroblast |
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| Source: | Organ: ovary Cell Type: fibroblast |
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| Permits/Forms: | In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location. | ||
| Isolation: | Isolation date: October, 1975 | ||
| Age: | juvenile | ||
| Comments: | The CCO cell line was derived from the ovaries of a healthy, juvenile channel catfish in October 1975 (PubMed: 7390534]. CCO is the cell line of choice for the propagation and diagnosis of Channel Catfish Virus (CCV). It is the standard for diagnosing Channel Catfish Virus Disease (CCVD) in farm reared Channel Catfish. The cells were cured of mycoplasma contamination in 1989 using Ciprofloxacin [J. Aquat. Anim. Health 6: 82-84, 1994]. | ||
| Propagation: | ATCC complete growth medium: The base medium for this cell line is ATCC-formulated Dulbecco's Modified Eagle's Medium, Catalog No. 30-2002. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%. Atmosphere: air, 95%; carbon dioxide (CO2), 5% Temperature: 30.0°C |
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| Subculturing: | Protocol:
Subcultivation Ratio: 1:4 to 1:6 weekly Medium Renewal: Two to three times weekly |
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| Preservation: | Freeze medium: Complete growth medium supplemented with 5% (v/v) DMSO Storage temperature: liquid nitrogen vapor phase |
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| Related Products: | Recommended medium (without the additional supplements or serum described under ATCC Medium):ATCC 30-2002 recommended serum:ATCC 30-2020 |
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| References: | 61176: Bowser PR, Plumb JA. Channel catfish virus: comparative replication and sensitivity of cell lines from channel catfish ovary and the brown bullhead. J. Wildl. Dis. 16: 451-454, 1980. PubMed: 7411751 61177: Bowser PR, Plumb JA. Fish cell lines: establishment of a line from ovaries of channel catfish. In Vitro 16: 365-368, 1980. PubMed: 7390534 70166: Hanson LA, Thune RL. Ciprofloxacin treatment eliminates Mycoplasma in contaminated channel catfish ovary cells. J. Aquat. Anim. Health 6: 82-84, 1994. |
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Channel Catfish Ovary [CCO]
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