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- 详细信息
- 文献和实验
- 技术资料
- 英文名:
Histopaque®-1077
- 供应商:
上海研卉生物
- 库存:
10771-100ML
- 规格:
100ml
sigma细胞分离液Histopaque®-1077
适用范围
Histopaque-1077 has been used for-
•外围血液单核细胞与骨髓单核细胞的分离
•通过离心纯化单核细胞
•人淋巴细胞分离
•从整个血液中分离乳腺癌循环肿瘤细胞
一般描述
Histopaque-1077 is a sterile,entoxin tested solution of polysucrose and diatrizoate,adjusted to a density of 1.077g/ml.This ready-to-use medium facilitates rapid recovery of available lymphocytes and other monuclear cells from small volumes of whole blo
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文献和实验了理论基础,而StemCell公司专利的四聚体复合物(TAC) 技术则为高纯度靶细胞的富集提供了保障。需要富集的靶细胞通过四聚体复合物与磁微粒相联结合,在EasySep® 专用磁极提供的磁场中,使靶细胞与非靶细胞分离。此方法即可用于正选,也可用于负选。EasySep®系统的优势为什么选用EasySep®?1、经济,应用正选抗体2、不需要柱子3、高纯度4、操作简单快速4、FACS兼容,磁珠很小不影响流式分析5、采用直接标记法分选人的细胞,采用间接标记法分选其它种类动物细胞独特的EasySep® 磁极
Lymphocyte Transformation 淋巴细胞转染
of histopaque -1077 into two other 50 ml tubes. Overlay the blood and wash media mixture onto the 10 ml of histopaque. Do this very slowly making sure not to mix the two layers.Centrifuge tubes for 30 minutes at 1500 rpm at room temperature (no brake), in the TJ
Method: Lymphocyte Transformation
-1077 into two other 50 ml tubes. Overlay the blood and wash media mixture onto the 10 ml of histopaque. Do this very slowly making sure not to mix the two layers. See figure 1, below. Centrifuge tubes for 30 minutes at 1500 rpm
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