BglII内切酶北京现货

特别提示：包括BglII内切酶在内，本公司的所有产品仅可用于科研实验，严禁用于临床医疗及其他非科研用途！

产品名称：BglII内切酶
英文名称：BglII
产品货号：GS2075
产品规格：500U

储存条件：冷冻（-20℃）

技术规格
 

Unit definition	One unit of the enzyme is the amount required to hydrolyze 1μgof Lambda DNA in 1hourat 37℃ in a total reaction volume of 50μl.
Recognition sites	A↑GATCT
来源	Isolated from an E.coli strain that carries the cloned Bgl II gene from Bacillus globigii
Assayed on	Lambda DNA
Optimal buffer	O(50mMTris-HCl(pH 7.6 at 25℃);10 mM MgCl2;100 mM NaCl;1 mM DTT.)
Optimal temperature	37℃
Storage conditions	10 mM Tris-HCl(pH 7.5);50mMNaCl;0.1 mM EDTA;7 mM 2-mercaptoethanol;200 μg/ml BSA;50%glycerol.Store at -20℃.
Ligations	After 10-fold overdigestion with enzyme more than 90%of the DNA fragments can be ligated and recut.
Non-specific hydrolisis	No nonspecific activity was detected after incubation of 1μgof Lambda DNA with 20 u.a.of enzyme for 16hoursat 37℃.
Methlation effect	Not blocked by overlapping dam methylation(GmATC)AGATCT
Thermal inactivation	enzyme is inactivated by incubation at 80℃ for 20 mins.

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