HindII内切酶优惠促销

特别提示：包括HindII内切酶在内，本公司的所有产品仅可用于科研实验，严禁用于临床医疗及其他非科研用途！

产品名称：HindII内切酶
英文名称：HindII
产品货号：GS2155
产品规格：500U

储存条件：冷冻（-20℃）

使用说明

To obtain 100%activity,BSA should be added to the 1 x reaction mix to a final concentration of 100μg/ml.Do not use BSA for long incubation.?

技术规格
 

Unit definition	One unit of the enzyme is the amount required to hydrolyze 1 μg of Lambda DNA in 1hourat 37℃ in a total reaction volume of 50μl.
Recognition sites	GTY↑RAC
来源	E.coli strain,that carries the cloned gene HindII from Haemophilus influenzae
Assayed on	Lambda DNA
Optimal buffer	G?(10mMTris-HCl(pH 7.6 at 25℃);10mMMgCl2;50 mM NaCl;1 mM DTT.)+?BSA
Optimal temperature	37℃
Storage conditions	10mMTris-HCl(pH 7.5);200 mM NaCl;0.1 mM EDTA;1 mM DTT;200 μg/ml BSA;50%glycerol.Store at -20℃.
Ligations	After 10-fold overdigestion with enzyme 60%of the DNA fragments can be ligated and recut.In the presence of 10%PEG ligation is better.
Non-specific hydrolisis	No nonspecific activity was detected after incubation of 1 μg of Lambda DNA with 20 u.a.of enzyme for 16hoursat 37℃.
Methlation effect	Methylation sensitivity not tested
Thermal inactivation	enzyme is inactivated by incubation at 65℃ for 20 mins.

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