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Amersham ECL Select是一种高灵敏度试剂，适合于化学发光蛋白免疫印迹检测。高信号灵敏度让Amersham ECL Select非常适合严苛的蛋白免疫印迹应用，能够检测出细微含量蛋白质。该试剂信号持续时间长，允许多次曝光，在实验和分析之间提供更方便的时间窗。信号可以使用CCD摄像机或X射线胶片检测。
对于以前熟悉Amersham ECL Advance的用户，Amersham ECL Select可以作为一项替代产品，它具有相同的灵敏度、信号强度和精确量化能力，适合苛刻的蛋白免疫印迹应用和细微含量蛋白质。
产品被引用文献：
A cancer stem cell-like phenotype is associated with miR-10b expression in aggressive squamous cell carcinomas，BY Monika Wimmer1†, Roland Zauner1†, Michael Ablinger1, Josefina Piñón-Hofbauer1, Christina Guttmann-Gruber1,Manuela Reisenberger1, Thomas Lettner1, Norbert Niklas2, Johannes Proell3, Mila Sajinovic4, Paul De Souza4,5,Stefan Hainzl1, Thomas Kocher1, Eva M. Murauer1, Johann W. Bauer1,6, Dirk Strunk7, Julia Reichelt1,
Albert Sleiman Mellick4,8 and Verena Wall,Wimmer et al. Cell Communication and Signaling (2020) 18:61
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文献和实验A cancer stem cell-like phenotype is associated with miR-10b expression in aggressive squamous cell carcinomas，BY Monika Wimmer1†, Roland Zauner1†, Michael Ablinger1, Josefina Piñón-Hofbauer1, Christina Guttmann-Gruber1,Manuela Reisenberger1, Thomas Lettner1, Norbert Niklas2, Johannes Proell3, Mila Sajinovic4, Paul De Souza4,5,Stefan Hainzl1, Thomas Kocher1, Eva M. Murauer1, Johann W. Bauer1,6, Dirk Strunk7, Julia Reichelt1,
Albert Sleiman Mellick4,8 and Verena Wall,Wimmer et al. Cell Communication and Signaling (2020) 18:61
https://doi.org/10.1186/s12964-020-00550-9
Western blotting using ECL reagent
diluted 1:8000 in TBS-T + 1.5% gelatin for 1 hour.6. Repeat step # 4 (wash)7. Prepare ECL solution for detection: mix equal volume of ECL reagent 1 and 2, (1:1) with final volume regarding of 0.125 ml/cm2 (for a mini gel- 4 ml of each reagent).8. Remove
temp for 1 hour. (Can be left at 4℃O/N here)►Wash briefly 2X then 3X10 min each with TBS-T►Mix an equal volume of detection reagent 1 with detection reagent 2 (usually 2mLs total is sufficient).►Drain excess TBS-T from membrane. Lay membrane on a piece
with 1 X PBST three times, each for 10 minutes.The Trident Universal Protein Blocking Reagent （animal serum-free） （GTX30963） does not contain any animal serum, and can be used for all IHC and ICC kits, ELISAs, and WBs.以 ECL 系统检测信号1. Follow the instructions
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