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奥林巴斯OLYMPUS
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荧光低倍变焦 MVX10
MVX10
研究型宏观变倍显微镜

An Intuitive Macro Fluorescence Imaging System
Designed for high fluorescence efficiency, the MVX10 stereo microscope offers flexibility for researchers interested in the impact of gene expression and protein function at the cellular level or within whole tissues, organs, and organisms.
Innovative Single-Zoom Bright Macro Fluorescence
The MVX10 MacroView, employs a single-zoom optical path with a large diameter, which is optimized to collect light with revolutionary efficiency and resolution at all magnifications. From fluorescent observation of whole organisms, such as zebrafish, at low magnification to the detailed observation of gene expression at the cellular level at high magnification, the MVX10 features a unique pupil division mechanism in the light-path to mimic the effect of a stereo microscope.


Seamless Observation from 4x to 125x
With the parfocal objectives and two-position revolving nosepiece, switching between objectives is very quick and easy, requiring little refocusing. Therefore, with the 0.63x and 2x objectives, the uninterrupted magnification range is 4x to 125x (eye observation with 10x eyepieces). This in combination with the 2x magnification change slider, increases effective magnification to 250X.
Contribution to Work Efficiency
This makes fluorescence screening and verification of gene expression especially efficient, improves speed and precision, reduces judgment errors, and eliminates the need to switch back and forth between a stereomicroscope and inverted microscope.
The peerless NA and S/N ratios of the optical components mean that specimens can be exposed to the excitation light for shorter periods. This is also true at near-infrared wavelengths, where the MVX10 has excellent transmission properties throughout the entire spectrum, minimizing damage to the specimen.

Choice of Suitable Contrast and Observation Method
SZX2-ILLTQ/SZX2-ILLTS
With a slim design new LED transmitted light illumination bases have a lower height to enable a low eyepoint and easy access to base-mounted samples during observation and operation. New LED illumination base SZX2-ILLTQ four-position turret (SZX2-ILLTS one –position) enables the user to choose cartridges and to switch from brightfield (Standard/High/Low), oblique (Standard/High/Low), darkfield, polarized illumination and shutter with a simple turn. This makes the MVX10 a flexible microscope for various samples and observation tasks. Another advantage of LED illumination is a cooler base surface, which is suitable for long duration manipulation of live specimens. Power consumption is lower than a conventional 30W halogen light source. A life cycle of over 60,000 hours significantly reduces operation costs.

C. elegans
Oblique high-contrast cartridge(SZX2-COBH)

Medaka
Darkfield cartridge(SZX2-CDF)
SZX2-STL
The SZX2-STL stand a stable, broad working space for observing large specimens. Attaching the Motorized Focus
Unit (SZX2-FOA) creates a more comfortable work environment.
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文献和实验2. 准备成像 设备接通电源后,需要花一些时间适当准备、放置和调平样品才能进行成像。 准备样品 首先,选择适当的盖玻片。盖玻片应为 1.5 或 0.17 毫米厚(170 微米)。配合盖玻片使用的奥林巴斯物镜需要合适的厚度才能获得良好的图像质量。如果盖玻片太厚或太薄,可能会出现光学伪影。 务必检查盖玻片的厚度,并使用标准载玻片。某些应用可以使用塑料载玻片。但对于荧光成像而言,塑料具有自发荧光特性。它会在蓝色和绿色通道(有时在红色通道)中产生明显的背景。 另外在成像之前清洁盖玻片和载玻片也非常重要。比较
。 免疫荧光染色不一定要用单抗,多抗也可以的。只要是可以用来做免疫组化的抗体都可以的——注意,有的抗体能用来做western bot,却不能做免疫组化,这一点要小心。 所用的二抗要求是抗-抗来源种属IgG的、用荧光染料标记的二抗。做完后要及时在与荧光染料对应的激发光下镜检并照相。 具体操作红皮书(分子生物学实验精编)上的“免疫细胞化学”一章节有介绍。 yongmingwu:原来我们科里照相用的都是普通相机,显微镜是Olympus Vanox的,现在想接一个数码相机来拍照。请问各位用过
,因为荧光激发块配置错误而得不到好的观察效果的现象经常发生,对于用户来说是一件非常烦恼的事。 为了方便用户正确选择激发和发射波长,节省时间,日本理化研究所脑科学所-奥林巴斯合作中心(the RIKEN BSI-Olympus Collaboration Center )开发了软件Spectrum Manager。用户选择荧光染料,软件会给出染料的光谱信息和推荐的滤色片选择等信息,使用非常方便。 还可利用Data Manager自行对光源信息、染料数据库或滤色片信息进行更新
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