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- 详细信息
- 文献和实验
- 技术资料
- 库存:
73
- 英文名:
RNase-Free DNase Set
- 保质期:
长期
- 供应商:
北京百奥莱博科技有限公司
- 保存条件:
冷藏(2-8℃)
- 规格:
50次|100次
概述
制备总RNA时,基因组DNA污染常常会干扰RT-PCR、Northern Blot等下游实验。本试剂盒是利用RNase-Free DNase I结合优化的吸附柱上反应体系,可在总RNA抽提过程中将残留的基因组DNA轻松清除,无需再进行酚氯仿抽提,使操作更方便、安全,最大限度避免RNA降解。纯化后的总RNA可以直接用于RNA印迹分析、斑点杂交、Poly(A)选择、体外翻译、RT-PCR分析、构建cDNA文库等RNA研究。
特点
- 试剂盒适用广泛,重复性好。
- 在吸附柱上将基因组DNA完全清除。
- 操作方便,避免RNA降解。
- 无需使用酚、氯仿等有毒试剂。
- 获得的总RNA无基因组DNA污染。
- 兼容性好,可与其它柱式总RNA抽提试剂盒配合使用。
应用
RT-PCR、Northern Blot、Poly A筛选、斑点杂交等
组份
RNase-Free DNase I(1U/μl);Buffer RDD;RNase-Free Water
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文献和实验How to Maintain an RNase-free Lab
How to Maintain an RNase-free Lab Let's admit it, everyone who has worked with RNA has, at some point, been affected by RNase contamination. Sources of contaminating RNase include procedures that use RNase such as plasmid preps
Ribonuclease A (RNase A), DNase-free
FeatureThe RNase A is free of DNase activity. It is not necessary to heat it before use.DescriptionThe Ribonuclease A (RNase A) is an endoribonuclease that specifically degrades single-stranded RNA at C and U residues. It cleaves the phosphodiester
Preparation of RNase-Free DNase by Alkylation
Characterization of RNA molecules by electrophoresis or hybridization frequently requires nucleic acid concentrations over 1 mg/mL. High molecular weight DNA in a mixture of nucleic acids limits the solubility and interferes
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