One unit is defined as the amount of enzyme that catalyzes the release of 60pmolof uracil per minute from double-stranded,uracil-containing DNA.Activity is measured by release of[3H]-uracil in a 50μlreaction containing 0,2μgDNA(104?~105?cpm/μg)in 30minutesat 37℃.
来源
Isolated from the recombinant source
Optimal buffer
SE-buffer Uracil DNA Glycosylase,(20mMTris-HCl(pH 8.0 at 25℃);1 mM EDTA;1 mM DTT)
Optimal temperature
37℃
Storage conditions
10 mM Tris-HCl(pH 7.6);50 mM NaCl;1 mM EDTA;1 mM DTT;50%glycerol.Store at -20℃.