BstSCI内切酶北京价格

特别提示：包括BstSCI内切酶在内，本公司的所有产品仅可用于科研实验，严禁用于临床医疗及其他非科研用途！

产品名称：BstSCI内切酶
英文名称：BstSCI
产品货号：GS2208
产品规格：100U

储存条件：冷冻（-20℃）

使用说明

At 37℃ activity is 10%from maximum.

技术规格
 

Unit definition	One unit of the enzyme is the amount required to hydrolyze 1μgof Lambda DNA(dcm-)in 1hourat 55℃ in a total reaction volume of 50μl.
Recognition sites	↑CCNGG
来源	Bacillus stearothermophilus SC
Assayed on	Lambda DNA(dcm-)
Optimal buffer	Y(33mMTris-acetate(pH 7.9 at 25℃);10 mM magnesium acetate;66 mM potassium acetate;1 mM DTT.)
Optimal temperature	55℃
Storage conditions	10 mM Tris-HCl(pH 7.5);50 mM KCl;0.1 mM EDTA;7 mM 2-mercaptoethanol;200 μg/ml BSA;and 50%glycerol.Store at -20℃.
Ligations	After 5-fold overdigestion with enzyme more than 95%of the DNA fragments can be ligated and recut.
Non-specific hydrolisis	No nonspecific activity was detected after incubation of 1μgof Lambda DNA with 3 u.a.of enzyme for 16hoursat 55℃.
Methlation effect	Blocked by Dcm-methylation(CmCWGG)?CCWGG.
Thermal inactivation	enzyme is inactivated by incubation at 80℃ for 20 mins.
Campatible Ends	Bme1390I^,BmrFI^,BssKI,MspR9I^,ScrFI^,StyD4I

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