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文献和实验1 A260 unit of duplex DNA = 50 ug 1 A260 unit of single-stranded DNA = 37 ug 1 A260 unit of single-stranded RNA = 50 ug 1 kb of DNA = 333 amino acids of coding capacity = 37,000 daltons Densities (50% GC
1 A260 unit of duplex DNA = 50 ug 1 A260 unit of single-stranded DNA = 37 ug 1 A260 unit of single-stranded RNA = 50 ug 1 kb of DNA = 333 amino acids of coding capacity = 37,000 daltons Densities (50% GC
STANDARD PLANT MOLECULAR BIOLOGY PROTOCOLS
and repeat step 3 5. Vortex the pellet, add 100 l MPS1 solution, vortex again and incubate the tubes at room temperature for 5 min 6. Add 20 l of a 20 mg/ml lysozyme solution, vortex-spin 1 sec and incubate 15 min at 37C. 7. Add 200 l MPS
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