- ¥853
- 赛默飞
- EF0651
- 2025年08月27日
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- 详细信息
- 文献和实验
- 技术资料
- 库存：
磷酸酶，激酶，FastAP Thermosensitive Alkaline Phosphatase (1 U/µL)
- 英文名：
磷酸酶，激酶，FastAP Thermosensitive Alkaline Phosphatase (1 U/µL)
- 规格：
1,000 units
| 磷酸酶&激酶 | EF0654 | FastAP Thermosensitive Alkaline Phosphatase (1 U/µL) | 300 units | ¥499 | ¥450 | |
| EF0651 | FastAP Thermosensitive Alkaline Phosphatase (1 U/µL) | 1,000 units | ¥853 | ¥750 | ||
| EF0652 | FastAP Thermosensitive Alkaline Phosphatase (1 U/µL) | 5 x 1,000 units | ¥3,432 | ¥2930 | ||
| EK0031 | T4 Polynucleotide Kinase (10 U/µL) | 500 units | ¥464 | ¥420 | ||
| EK0032 | T4 Polynucleotide Kinase (10 U/µL) | 2,500 units | ¥1,847 | ¥1590 |
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文献和实验, incubate for 30 min at 37°C. ( 5g powdered milk in 95 ml PBS)5 Rinse with PBS 3x.6 Add 50 µl of antibody, incubate 30 min at 37°C.7 Rinse with PBS 3x.8 Add 50 µl of goat anti-mouse IgG conjugate with alkaline phosphatase.(1 µl of conjugate in 4 ml of PBS
ELISA Procedure for Measuring Serum Antibody Titer
IgG/alkaline phosphatase conjugate (usually 1:1000 or greater) that is appropriate to the source of your antibodies in PBS-T. Pipette 200 µL of this solution into each of the wells and incubate for three hours at 37 °C or overnight at 2-6 °C
Protocol for 5' End Labeling RNA
10X Dephosphorylation Buffer (0.5 M Tris, pH 8.5, 1 mM EDTA, pH 8) 1 µl Calf Intestinal Phosphatase (CIP; 0.1 U/µl) 2) Incubate 1 hr at 37°C. Remove the Calf Intestine Alkaline Phosphatase by use of the Phosphatase Removal Reagent
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