- ￥1350 - 3000
- Proteintech
- 66422-1-Ig
- 2025年10月13日
- WB, IHC, IF/ICC, ELISA
- Mouse
- human, mouse, rat, pig
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- 详细信息
- 文献和实验
- 技术资料
- 免疫原：
Recombinant human PDI protein
- 亚型：
IgG2b
- 保存条件：
Store at -20°C. Stable for one year after shipment.
- 克隆性：
Monoclonal
- 标记物：
Unconjugated
- 适应物种：
human, mouse, rat, pig
- 保质期：
详见产品包装
- 库存：
充足
- 宿主：
Mouse
- 应用范围：
WB, IHC, IF/ICC, ELISA
- 靶点：
PDI
- 抗体英文名：
PDI Monoclonal antibody
- 抗体名：
PDI Monoclonal antibody
- 规格：
50ul/100ul/150ul
| 规格： | 50ul | 产品价格： | ￥1350.0 |
|---|---|---|---|
| 规格： | 100ul | 产品价格： | ￥2200.0 |
| 规格： | 150ul | 产品价格： | ￥3000.0 |
经过测试的应用
| Positive WB detected in | HEK-293 cells, rat brain tissue, HepG2 cells, L02 cells, COLO 320 cells |
| Positive IHC detected in | human liver tissue, human small intestine tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | HepG2 cells |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:10000-1:100000 |
| Immunohistochemistry (IHC) | IHC : 1:100-1:400 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:200-1:800 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
66422-1-Ig targets PDI in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat, pig samples.
| 经测试应用 | WB, IHC, IF/ICC, ELISA |
| 文献引用应用 | WB, IF |
| 经测试反应性 | human, mouse, rat, pig |
| 文献引用反应性 | human, mouse, bovine |
| 免疫原 |
CatNo: Ag1747 Product name: Recombinant human PDI protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 89-273 aa of BC014504 Sequence: MTKYKPESEELTAEWITEFCHRFLEGKIKPHLMSQELPEDWDKQPVKVLVGKNFEDVAFDEKKNVFVEFYAPWCGHCKQLAPIWDKLGETYKDHENIVIAKMDSTANEVEAVKVHSFPTLKFFPASADRTVIDYNGERTLDGFKKFLESGGQDGAGDDDDLEDLEEAEEPDMEEDDDQKAVKDEL |
| 宿主/亚型 | Mouse / IgG2b |
| 抗体类别 | Monoclonal |
| 产品类型 | Antibody |
| 全称 | prolyl 4-hydroxylase, beta polypeptide |
| 别名 | P4HB, ERBA2L, EC:5.3.4.1, DSI, CLCRP1 |
| 计算分子量 | 57 kDa |
| 观测分子量 | 57 kDa |
| GenBank蛋白编号 | BC014504 |
| 基因名称 | PDI |
| Gene ID (NCBI) | 5034 |
| RRID | AB_2881794 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Protein A purification |
| UNIPROT ID | P07237 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
PDIA1(Protein disulfide-isomerase) is also named as ERBA2L, PDI, P4HB, PO4DB. It is a multifunctional protein that catalyzes the formation, breakage and rearrangement of disulfide bonds. In some cell types, it seems to be secreted or associated with the plasma membrane, where it undergoes constant shedding and replacement from intracellular sources.It can exsit as homodimer and monomers and homotetramers may also occur(PMID:12095988).
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文献和实验| Species | Application | Title |
|---|---|---|
| bovine | IF | J Agric Food Chem Acylated Ghrelin Activates PI3K/mTOR Signaling Pathway by Promoting ThPOK Acetylation to Promote Milk Fat Synthesis in Bovine Mammary Epithelial CellsAuthors - Jiaxin Wang |
| human | IF | Biochim Biophys Acta Mol Cell Res SLC35A2 deficiency reduces protein levels of core 1 β-1,3-galactosyltransferase 1 (C1GalT1) and its chaperone Cosmc and affects their subcellular localizationAuthors - Wojciech Wiertelak |
| human | IF | J Cell Sci A general role for TANGO1, encoded by MIA3, in secretory pathway organization and functionAuthors - Janine McCaughey |
| human | WB | Mol Pharm Highly Efficient Method for Intracellular Delivery of Proteins Mediated by Cholera Toxin-Induced Protein Internalization.Authors - Xiaofan Jia |
| human | IF | Cell Signal HRD1-mediated PTEN degradation promotes cell proliferation and hepatocellular carcinoma progression.Authors - Liu Liu |
| human | IF | Biochim Biophys Acta Gen Subj Expression of GALNT8 and O-glycosylation of BMP receptor 1A suppress breast cancer cell proliferation by upregulating ERα levelsAuthors - Tianmiao Huang |
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