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- 文献和实验
- 技术资料
- 免疫原:
None
- 亚型:
IgG1, kappa
- 保存条件:
Store at 2-8°C. Avoid exposure to light. Stable for one year after shipment.
- 克隆性:
Monoclonal
- 标记物:
APC Fluorescent Dye
- 适应物种:
Human
- 保质期:
详见产品包装
- 库存:
充足
- 宿主:
Mouse
- 应用范围:
FC
- 靶点:
CD8a
- 抗体英文名:
APC Anti-Human CD8a (Hit8a)
- 抗体名:
APC Anti-Human CD8a (Hit8a)
- 规格:
25tests/100tests
| 规格: | 25tests | 产品价格: | ¥600.0 |
|---|---|---|---|
| 规格: | 100tests | 产品价格: | ¥1200.0 |
经过测试的应用
| Positive FC detected in | human PBMCs |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Flow Cytometry (FC) | FC : 5 ul per 10^6 cells in 100 μl suspension |
| This reagent has been pre-titrated and tested for flow cytometric analysis. The suggested use of this reagent is 5 μl per 10^6 cells in a 100 µl suspension or 5 μl per 100 µl of whole blood. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
APC-65113 targets CD8a in FC applications and shows reactivity with Human samples.
| 经测试应用 | FC |
| 经测试反应性 | Human |
| 免疫原 |
fusion protein |
| 宿主/亚型 | Mouse / IgG1, kappa |
| 抗体类别 | Monoclonal |
| 产品类型 | Antibody |
| 全称 | CD8a molecule |
| 别名 | CD8, CD8A, CD8a molecule, Leu2, MAL, p32 |
| 计算分子量 | 235 aa, 26 kDa |
| GenBank蛋白编号 | BC025715 |
| 基因名称 | CD8A |
| Gene ID (NCBI) | 925 |
| ENSEMBL Gene ID | ENSG00000153563 |
| RRID | AB_2883002 |
| 偶联类型 | APC Fluorescent Dye |
| 最大激发/发射波长 | 650 nm / 660 nm |
| 形式 | Liquid |
| 纯化方式 | Affinity purification |
| UNIPROT ID | P01732 |
| 储存缓冲液 | PBS with 0.09% sodium azide and 0.5% BSA, pH 7.3. |
| 储存条件 | Store at 2-8°C. Avoid exposure to light. Stable for one year after shipment. |
背景介绍
CD8 is a transmembrane glycoprotein composed of two disulfide-linked chains. It can be present as a homodimer of CD8α or as a heterodimer of CD8α and CD8β (PMID: 3264320; 8253791). CD8 is found on most thymocytes. The majority of class I-restricted T cells express mostly the CD8αβ heterodimer while CD8αα homodimers alone have been found on some gut intraepithelial T cells , on some T cell receptor (TCR) γδ T cells and on NK cells (PMID: 2111591; 1831127; 8420975). CD8 acts as a co-receptor that binds to MHC class-I and participates in cytotoxic T cell activation (PMID: 8499079). During T cell development, CD8 is required for positive selection of CD4-/CD8+ T cells (PMID: 1968084).
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结直肠癌 (CRC) 是胃肠道中常见的恶性肿瘤,其形成也是一个逐步发展的过程。前期研究数据表明,大多数结直肠癌起始于异常的腺窝,后逐渐演变成息肉,最终发展为结肠直肠癌,整个过程可长达 5 - 15 年。在内在分子机制层面,大多数结直肠癌是通过 WNT 通路的永久激活而启动的,而这种激活通常是发生在肠道干细胞中肿瘤抑制基因 APC 的突变之后。 APC 的突变可诱导肠内细胞增殖增加,防止细胞死亡并阻断分化,所有这些特征均可能有助于提高 APC 突变细胞的相对适应度,APC 突变的肠道干细胞及其后
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