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- 详细信息
- 文献和实验
- 技术资料
- 免疫原:
None
- 亚型:
IgG2b
- 保存条件:
Store at -20°C. Stable for one year after shipment.
- 克隆性:
Monoclonal
- 标记物:
Unconjugated
- 适应物种:
human
- 保质期:
详见产品包装
- 库存:
充足
- 宿主:
Mouse
- 应用范围:
WB, IHC, IF-P, ELISA
- 靶点:
Beta-2-Microglobulin
- 抗体英文名:
Beta-2-Microglobulin Monoclonal antibody
- 抗体名:
Beta-2-Microglobulin Monoclonal antibody
- 规格:
50ul/100ul/150ul
| 规格: | 50ul | 产品价格: | ¥1350.0 |
|---|---|---|---|
| 规格: | 100ul | 产品价格: | ¥2200.0 |
| 规格: | 150ul | 产品价格: | ¥3000.0 |
经过测试的应用
| Positive WB detected in | A431 cells, Raji cells, LNCaP cells |
| Positive IHC detected in | human prostate cancer tissue, human renal cell carcinoma tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF-P detected in | human prostate cancer tissue |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:2000-1:10000 |
| Immunohistochemistry (IHC) | IHC : 1:250-1:1000 |
| Immunofluorescence (IF)-P | IF-P : 1:200-1:800 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
66207-1-Ig targets Beta-2-Microglobulin in WB, IHC, IF-P, ELISA applications and shows reactivity with human samples.
| 经测试应用 | WB, IHC, IF-P, ELISA |
| 文献引用应用 | WB |
| 经测试反应性 | human |
| 文献引用反应性 | human, mouse, rat, monkey |
| 免疫原 |
fusion protein |
| 宿主/亚型 | Mouse / IgG2b |
| 抗体类别 | Monoclonal |
| 产品类型 | Antibody |
| 全称 | beta-2-microglobulin |
| 别名 | B2M, 1C3B7, HDCMA22P, CDABP0092, Beta-2-microglobulin form pI 5.3 |
| 计算分子量 | 14 kDa |
| 观测分子量 | 13 kDa |
| GenBank蛋白编号 | BC032589 |
| 基因名称 | B2M |
| Gene ID (NCBI) | 567 |
| ENSEMBL Gene ID | ENSG00000166710 |
| RRID | AB_2881598 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Protein A purification |
| UNIPROT ID | P61769 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
Beta-2-microglobulin (B2M) is a component of MHC class I molecules, which are present on the surface of nearly all nucleated cells. It can be found in body fluids under physiologic conditions as a result of shedding from cell surfaces or intracellular release. B2M has various biological functions, including antigen presentation. Investigations reveal that increased synthesis and release of B2M are present in several malignant diseases.
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文献和实验| Species | Application | Title |
|---|---|---|
| mouse | WB | J Immunother Cancer Targeting oxidative phosphorylation to increase the efficacy of immune-combination therapy in renal cell carcinomaAuthors - Jihua Tian |
| human | WB | J Agric Food Chem Carnosic Acid: A Novel Selective Inhibitor of ERAP1 by Direct Binding and Its Modulation of Antigen Processing and PresentationAuthors - Jiaqi Wu |
| monkey | WB | J Virol The neonatal Fc receptor (FcRn) is required for porcine reproductive and respiratory syndrome virus uncoatingAuthors - Kang Yang |
| rat | WB | Int Immunopharmacol Identification of potential targets regulating neutrophil extracellular traps in acute rejection of kidney transplantation based on transcriptomics and animal experimentsAuthors - Jun Pei |
抗体的检测外,还应该用与其抗原成分相关的其它抗原进行交叉试验,方法可用ELISA、IFA法。例如:①制备抗黑色素瘤细胞的McAb,除用黑色素瘤细胞反应外,还应该用其它脏器的肿瘤细胞和正常细胞进行交叉反应,以便挑选肿瘤特异性或肿瘤相关抗原的单克隆抗体。②制备抗重组的细胞因子的单克隆抗体,应首先考虑是否与表达菌株的蛋白有交叉反应,其次是与其它细胞因子间有无交叉。 2.McAb的Ig类与亚类的鉴定 一般在用酶标或荧光素标记的第二抗体进行筛选时已经基本上确定了抗体的Ig类型
免疫系统受抗原刺激后,B细胞转化为浆细胞,由浆细胞产生与抗原发生特异性结合的球蛋白,这类球蛋白称为抗体(antibody,Ab)。 免疫球蛋白(immunoglobulim,Ig)是指具有抗体活性或化学结构与抗体相似的球蛋白。 免疫球蛋白普遍存在于哺乳动物和人类的血液、组织液和外分泌中。人体的免疫球蛋白主要有五类:IgM、IgA、IgG、IgD、IgE。 (一) 抗体结构 抗体是具有4条多肽链的对称
(一)Ig超家族的结构特点 Ig超家族成员均含有1~7个Ig样结构域,每个Ig样结构域约含70~110个氨基酸残基。其二级结构是两个各含3~5个反平行β折叠股所形成的β片层(anti-parallel β-pleated sheet)平面,每个反平行β折叠股由5~10个氨基酸残基组成,β片层内侧的疏水性氨基酸起到稳定Ig折叠
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