- ￥1350 - 3000
- Proteintech
- 22336-1-AP
- 2025年10月13日
- WB, IP, IHC, ELISA
- Rabbit
- human
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- 详细信息
- 文献和实验
- 技术资料
- 免疫原：
Recombinant human ABCB1 protein
- 亚型：
IgG
- 保存条件：
Store at -20°C. Stable for one year after shipment.
- 克隆性：
Polyclonal
- 标记物：
Unconjugated
- 适应物种：
human
- 保质期：
详见产品包装
- 库存：
充足
- 宿主：
Rabbit
- 应用范围：
WB, IP, IHC, ELISA
- 靶点：
P glycoprotein
- 抗体英文名：
P glycoprotein Polyclonal antibody
- 抗体名：
P glycoprotein Polyclonal antibody
- 规格：
50ul/100ul/150ul
| 规格： | 50ul | 产品价格： | ￥1350.0 |
|---|---|---|---|
| 规格： | 100ul | 产品价格： | ￥2200.0 |
| 规格： | 150ul | 产品价格： | ￥3000.0 |
经过测试的应用
| Positive WB detected in | unboiled HeLa cells, unboiled HepG2 cells |
| Positive IP detected in | human placenta tissue |
| Positive IHC detected in | human liver cancer tissue, human kidney tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Boiling the sample after lysis is not recommended.
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:500-1:1000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
22336-1-AP targets P glycoprotein in WB, IHC, IF, IP, CoIP, ELISA applications and shows reactivity with human samples.
| 经测试应用 | WB, IP, IHC, ELISA |
| 文献引用应用 | WB, IHC, IF, IP, CoIP |
| 经测试反应性 | human |
| 文献引用反应性 | human, mouse, rat, pig |
| 免疫原 |
CatNo: Ag17811 Product name: Recombinant human ABCB1 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 624-708 aa of BC130424 Sequence: KLVTMQTAGNEVELENAADESKSEIDALEMSSNDSRSSLIRKRSTRRSVRGSQAQDRKLSTKEALDESIPPVSFWRIMKLNLTEW |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | ATP-binding cassette, sub-family B (MDR/TAP), member 1 |
| 别名 | ABC20, ABCB1, CD243, CLCS, GP170, MDR1, Multidrug resistance protein 1, P glycoprotein, P glycoprotein 1, P-gp |
| 计算分子量 | 1280 aa, 142 kDa |
| 观测分子量 | 130-150 kDa |
| GenBank蛋白编号 | BC130424 |
| 基因名称 | P glycoprotein |
| Gene ID (NCBI) | 5243 |
| RRID | AB_2833023 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | P08183 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
ABCB1 (also known as P-gp/ P-glycoprotein, MDR1/multidrug-resistance 1) is a plasma membrane protein first discovered in multidrug resistant tumor cells. ABCB1 can extrude a large number of medically relevant compounds from cells and causes drug resistance. ABCB1 is expressed in a variety of human cancers as well as normal tissues including brain, and placenta. Overexpression of ABCB1 correlates with a negative prognosis in several types of cancer. For optimal WB detection with 22336-1-AP, we recommend to avoid boiling the sample after lysis.
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文献和实验| Species | Application | Title |
|---|---|---|
| human | WB | Drug Resist Updat Cell membrane-camouflaged bufalin targets NOD2 and overcomes multidrug resistance in pancreatic cancerAuthors - Wei Zhang |
| human,mouse | WB,IF | Drug Resist Updat Lansoprazole (LPZ) reverses multidrug resistance (MDR) in cancer through impeding ATP-binding cassette (ABC) transporter-mediated chemotherapeutic drug efflux and lysosomal sequestrationAuthors - Ning Ji |
| human | WB,IHC | Mol Cancer Hsa-miR-3178/RhoB/PI3K/Akt, a novel signaling pathway regulates ABC transporters to reverse gemcitabine resistance in pancreatic cancer.Authors - Jianyou Gu |
| human | WB | Mol Cancer PMID: 32727463Authors - Cefan Zhou |
| human | WB | J Control Release cRGD-targeted heparin nanoparticles for effective dual drug treatment of cisplatin-resistant ovarian cancerAuthors - Xiaomei Liang |
| human | WB | ACS Appl Mater Interfaces TPGS-Galactose-Modified Polydopamine Co-delivery Nanoparticles of Nitric Oxide Donor and Doxorubicin for Targeted Chemo-Photothermal Therapy against Drug-Resistant Hepatocellular Carcinoma.Authors - Zijing Du |
通路是通过磷酸化激活下游的，磷酸化的就是活化形式啦，比如c-jun吧，有钱的话还是要要买c-jun和p-c-jun的,紧张的话但用p-c-jun也能说明问题，里面的各型无非就是多抗，兔或鼠单抗，兔单抗比较好，选择的话还要看你做什么用途，个人浅见 chuckdouble superman211 wrote: 拟通过WB进行AP-1的相关研究，在选择一抗时遇到如下困惑，望了解的战友予以解答。关于AP-1的出c-jun的一抗有多种，其中主要
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