- ￥1350 - 3000
- Proteintech
- 18262-1-AP
- 2025年10月13日
- WB, IHC, IP, ELISA
- Rabbit
- human, mouse, rat
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- 详细信息
- 文献和实验
- 技术资料
- 免疫原：
Recombinant human PDK1 protein
- 亚型：
IgG
- 保存条件：
Store at -20°C. Stable for one year after shipment.
- 克隆性：
Polyclonal
- 标记物：
Unconjugated
- 适应物种：
human, mouse, rat
- 保质期：
详见产品包装
- 库存：
充足
- 宿主：
Rabbit
- 应用范围：
WB, IHC, IP, ELISA
- 靶点：
PDK1
- 抗体英文名：
PDK1 Polyclonal antibody
- 抗体名：
PDK1 Polyclonal antibody
- 规格：
50ul/100ul/150ul
| 规格： | 50ul | 产品价格： | ￥1350.0 |
|---|---|---|---|
| 规格： | 100ul | 产品价格： | ￥2200.0 |
| 规格： | 150ul | 产品价格： | ￥3000.0 |
经过测试的应用
| Positive WB detected in | mouse heart tissue, HEK-293 cells, NIH/3T3 cells, mouse liver tissue, rat liver tissue |
| Positive IP detected in | SiHa cells |
| Positive IHC detected in | human liver cancer tissue, human heart tissue, human kidney tissue, human breast cancer tissue, mouse kidney tissue, rat kidney tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:6000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:500-1:2000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
18262-1-AP targets PDK1 in WB, IHC, IF, IP, ELISA applications and shows reactivity with human, mouse, rat samples.
| 经测试应用 | WB, IHC, IP, ELISA |
| 文献引用应用 | WB, IHC, IF |
| 经测试反应性 | human, mouse, rat |
| 文献引用反应性 | human, mouse, rat |
| 免疫原 |
CatNo: Ag13121 Product name: Recombinant human PDK1 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-436 aa of BC039158 Sequence: MRLARLLRGAALAGPGPGLRAAGFSRSFSSDSGSSPASERGVPGQVDFYARFSPSPLSMKQFLDFGSVNACEKTSFMFLRQELPVRLANIMKEISLLPDNLLRTPSVQLVQSWYIQSLQELLDFKDKSAEDAKAIYDFTDTVIRIRNRHNDVIPTMAQGVIEYKESFGVDPVTSQNVQYFLDRFYMSRISIRMLLNQHSLLFGGKGKGSPSHRKHIGSINPNCNVLEVIKDGYENARRLCDLYYINSPELELEELNAKSPGQPIQVVYVPSHLYHMVFELFKNAMRATMEHHANRGVYPPIQVHVTLGNEDLTVKMSDRGGGVPLRKIDRLFNYMYSTAPRPRVETSRAVPLAGFGYGLPISRLYAQYFQGDLKLYSLEGYGTDAVIYIKALSTDSIERLPVYNKAAWKHYNTNHEADDWCVPSREPKDMTTFRSA |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | pyruvate dehydrogenase kinase, isozyme 1 |
| 别名 | [Pyruvate dehydrogenase (acetyl-transferring)] kinase isozyme 1, mitochondrial, EC:2.7.11.2, PDH kinase 1, PDHK1 |
| 计算分子量 | 436 aa, 49 kDa |
| 观测分子量 | 46-55 kDa |
| GenBank蛋白编号 | BC039158 |
| 基因名称 | PDK1 |
| Gene ID (NCBI) | 5163 |
| RRID | AB_10598310 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | Q15118 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
PDK1(pyruvate dehydrogenase kinase isoform 1) is also named as PDHK1 and belongs to the PDK/BCKDK protein kinase family. It inhibits the mitochondrial pyruvate dehydrogenase complex by phosphorylation of the E1 alpha subunit, thus contributing to the regulation of glucose metabolism (PMID:17683942). Starvation increases islet PDK activity (PMID:11723055). PDK1 has 2 isoforms with the MW of 49 and 52 kD, and a 28aa-transit-peptide in the N-terminal can be removed in the mature form. This antibody is specific to PDK1.
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文献和实验| Species | Application | Title |
|---|---|---|
| human,mouse | WB,IHC | J Clin Invest PRMT5 mediated FUBP1 methylation accelerates prostate cancer progressionAuthors - Weiwei Yan |
| mouse | WB,IF | Cardiovasc Res Inhibition of PGK1 attenuates autoimmune myocarditis by reprogramming CD4+ T cells metabolismAuthors - Yang Lu |
| mouse | WB,IF | Cell Death Dis Pyruvate dehydrogenase kinase 1 protects against neuronal injury and memory loss in mouse models of diabetesAuthors - Yuan Yao |
| mouse | WB | Bioact Mater Polydopamine-mediated graphene oxide and nanohydroxyapatite-incorporated conductive scaffold with an immunomodulatory ability accelerates periodontal bone regeneration in diabetes.Authors - Yazhen Li |
| human | WB,IHC | Cancer Lett PINK1 deficiency in gastric cancer compromises mitophagy, promotes the Warburg effect, and facilitates M2 polarization of macrophages.Authors - Ying Xu |
| human | WB | Redox Biol Impairment of Akt activity by CYP2E1 mediated oxidative stress is involved in chronic ethanol-induced fatty liver.Authors - Tao Zeng |
因此，综合来说，EarthOx的GST标签抗体 （货号#E022040）是一款同时具有高特异性、高灵敏度、多应用类型以及性价比的GST标签抗体。同时，我们也提供其他品牌的GST标签抗体，如Proteintech的GST多克隆抗体 ――GST PolyClonal Antibody（货号#10000-0-AP）以及Abcam、Santa等多种GST标签抗体产品。更多GST标签抗体以及其他如His、HA、Myc、GFP、Flag等其他标签抗体，请电话垂询。美国EarthOx品牌是一家提供高性价比免疫学和细胞
实验，请根据说明书列出已验证的种属来选择适合你实验的抗体。 （4）样本蛋白的结构性质。了解样本蛋白的结构性质有助于选择最合适的抗体，待测样本蛋白的结构域和样本在提取和处理过程中是否会变性，蛋白空间构象的改变，会影响抗体的免疫亲和反应。 （5）单多克隆抗体的选择。市面上的抗体还是以多克隆抗体为主，一般单克隆抗体特异性强，但亲和力相对小，检测抗原灵敏度相对就低；而多克隆抗体特异性稍弱，但抗体的亲和力强，灵敏度高，但易出现非特异性染色（可以通过封闭等避免
发生，最好在特异性抗体处理切片之前，选择与二抗种属相同的非免疫血清封闭电荷，阻止一抗与之结合，抑制非特异性背景着色。常用方法是用2%～10%羊血清或2%～5%牛血清白蛋白在室温下作用10~30分钟。但应注意此种结合不牢固，所以最好不要冲洗，倾去余液直接加一抗。SABC或SP免疫组织化学试剂盒中常配有非免疫血清。 7．第一抗体孵育 滴加特异性一抗于切片上，4℃孵育24～48小时，或室温孵育过夜，也可在37~C孵育1～2小时。之后，用PBS冲洗3次，每次5分钟。一抗分为多克隆抗体和单克
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