- ￥1350 - 3000
- Proteintech
- 17490-1-AP
- 2025年10月13日
- WB, IHC, IF/ICC, IF-P, IF-Fro, FC (Intra), IP, ELISA
- Rabbit
- human, mouse, rat
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- 详细信息
- 文献和实验
- 技术资料
- 免疫原：
Recombinant human MAP2 protein
- 亚型：
IgG
- 保存条件：
Store at -20°C. Stable for one year after shipment.
- 克隆性：
Polyclonal
- 标记物：
Unconjugated
- 适应物种：
human, mouse, rat
- 保质期：
详见产品包装
- 库存：
充足
- 宿主：
Rabbit
- 应用范围：
WB, IHC, IF/ICC, IF-P, IF-Fro, FC (Intra), IP, ELISA
- 靶点：
MAP2
- 抗体英文名：
MAP2 Polyclonal antibody
- 抗体名：
MAP2 Polyclonal antibody
- 规格：
50ul/100ul/150ul
| 规格： | 50ul | 产品价格： | ￥1350.0 |
|---|---|---|---|
| 规格： | 100ul | 产品价格： | ￥2200.0 |
| 规格： | 150ul | 产品价格： | ￥3000.0 |
经过测试的应用
| Positive WB detected in | SH-SY5Y cells, rat brain tissue, mouse brain tissue |
| Positive IP detected in | SH-SY5Y cells, mouse brain tissue |
| Positive IHC detected in | mouse brain tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF-P detected in | rat brain tissue, mouse brain tissue |
| Positive IF-Fro detected in | mouse brain tissue, rat brain tissue |
| Positive IF/ICC detected in | iPS cells |
| Positive FC (Intra) detected in | Neuro-2a cells |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:5000-1:50000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:2500-1:10000 |
| Immunofluorescence (IF)-P | IF-P : 1:50-1:500 |
| Immunofluorescence (IF)-FRO | IF-FRO : 1:50-1:500 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:125-1:500 |
| Flow Cytometry (FC) (INTRA) | FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
17490-1-AP targets MAP2 in WB, IHC, IF/ICC, IF-P, IF-Fro, FC (Intra), IP, ELISA applications and shows reactivity with human, mouse, rat samples.
| 经测试应用 | WB, IHC, IF/ICC, IF-P, IF-Fro, FC (Intra), IP, ELISA |
| 文献引用应用 | WB, IHC, IF |
| 经测试反应性 | human, mouse, rat |
| 文献引用反应性 | human, mouse, rat, monkey, goat |
| 免疫原 |
CatNo: Ag11580 Product name: Recombinant human MAP2 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 213-559 aa of BC038857 Sequence: TSAGSTDRLPYSKSGNKDGVTKSPEKRSSLPRPSSILPPRRGVSGDRDENSFSLNSSISSSARRTTRSEPIRRAGKSGTSTPTTPGSTAITPGTPPSYSSRTPGTPGTPSYPRTPHTPGTPKSAILVPSEKKVAIIRTPPKSPATPKQLRLINQPLPDLKNVKSKIGSTDNIKYQPKGGQVRILNKKIDFSKVQSRCGSKDNIKHSAGGGNVQIVTKKIDLSHVTSKCGSLKNIRHRPGGGRVKIESVKLDFKEKAQAKVGSLDNAHHVPGGGNVKIDSQKLNFREHAKARVDHGAEIITQSPGRSSVASPRRLSNVSSSGSINLLESPQLATLAEDVTAALAKQGL |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | microtubule-associated protein 2 |
| 别名 | MAP 2, MAP-2, MAP2A, MAP2B, MAP2C |
| 计算分子量 | 200 kDa |
| 观测分子量 | 280 kDa, 70-85 kDa |
| GenBank蛋白编号 | BC038857 |
| 基因名称 | MAP2 |
| Gene ID (NCBI) | 4133 |
| RRID | AB_2137880 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | P11137 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
Microtubule-associated protein 2 (MAP2) is a tubulin binding protein regulating the spacing and stability of microtubules and contributing to elongation of dendrites.
What is the molecular weight of MAP2? Is MAP2 post-translationally modified?
MAP2 has multiple isoforms that arise from alternative splicing (PMID: 3121794, 7854050, and 10383434). They are classified into two groups - MAP2A and MAP2B, which are known as high molecular weight (HMW) isoforms, run as ~280 kDa species, while low molecular weight (LMW) isoforms MAP2C and MAP2D are around ~70 kDa. MAP2 proteins are heavily phosphorylated, which contributes to a large discrepancy between their predicted and observed molecular weight in SDS-PAGE (220 vs 280 kDa for HMW forms).
What is the tissue expression pattern of MAP2? What is the subcellular localization of MAP2?
MAP2 isoforms differ in their tissue and developmental expression pattern (PMID: 2469170 and 3898077). In the brain, MAP2B is widely expressed during and post development, MAP2A is expressed postnatally, while MAP2C is present only in the early development except of present in photosensitive cells of the adult retina and in the olfactory system. MAP proteins are highly expressed in the CNS found in cell bodies and dendrites of neurons, in dorsal root ganglion, reactive glia, and in the testis (PMID: 9588626). In neurons, MAP2 proteins are found in the cell body and dendrites, where they associate with microtubules, while they can also be present in the nuclei of testicular cells.
Can MAP2 be used as a neuronal marker?
MAP2 proteins are abundantly expressed in neurons. MAP2 is frequently used as a dendritic marker because it is present in the cell body and dendrites of neurons but absent in axons (PMID: 28413822).
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文献和实验| Species | Application | Title |
|---|---|---|
| mouse | IF | Cell Stem Cell In vivo reprogramming of NG2 glia enables adult neurogenesis and functional recovery following spinal cord injury.Authors - Wenjiao Tai |
| human | IF | Cell Stem Cell Recent Zika Virus Isolates Induce Premature Differentiation of Neural Progenitors in Human Brain Organoids.Authors - Elke Gabriel |
| human | IF | Nat Commun Reliability of high-quantity human brain organoids for modeling microcephaly, glioma invasion and drug screeningAuthors - Anand Ramani |
| mouse | WB,IF | Neuron Astrocytic ApoE reprograms neuronal cholesterol metabolism and histone-acetylation-mediated memory.Authors - Xiaohui Li |
Dev Cell Nucleoporin Seh1 controls murine neocortical development via transcriptional repression of p21 in neural stem cellsAuthors - Wenxiu Dai | ||
| mouse | WB | Brain Reactive microglia enhance the transmission of exosomal alpha-synuclein via toll-like receptor 2.Authors - Yun Xia |
至胞核，形成转录因子AP-1。 其中MAPK由MAPK的激酶(MAP2K)活化，而MAP2K又由MAP3K活化，构成级联反应。注意在两条相关信号途径中(绿色和蓝色)组成各激酶的成员并不相同。
3K，详下)，再由Raf顺序激活MAP激酶家族成员Erk(extra-cellular signal-regulated protein kinase)。后者引起另一个重要的转录因子Ap-l的组成成分Pos和Jun磷酸化，并发生转位。但实际上，癌基因产物Fos和Jun可能是在细胞核内被激活的，因而MAP激酶具有进入细胞核，使底物发生磷酸化的作用。 Ras-GDP和Ras-GTP的转换及其调节 无活性的Ras-GDP在鸟苷酸置换因子sos的作用下脱去GDP并以GTP的取代后形成有活性
因此，综合来说，EarthOx的GST标签抗体 （货号#E022040）是一款同时具有高特异性、高灵敏度、多应用类型以及性价比的GST标签抗体。同时，我们也提供其他品牌的GST标签抗体，如Proteintech的GST多克隆抗体 ――GST PolyClonal Antibody（货号#10000-0-AP）以及Abcam、Santa等多种GST标签抗体产品。更多GST标签抗体以及其他如His、HA、Myc、GFP、Flag等其他标签抗体，请电话垂询。美国EarthOx品牌是一家提供高性价比免疫学和细胞
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