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- 详细信息
- 文献和实验
- 技术资料
- 免疫原:
Recombinant human Albumin protein
- 亚型:
IgG
- 保存条件:
Store at -20°C. Stable for one year after shipment.
- 克隆性:
Polyclonal
- 标记物:
Unconjugated
- 适应物种:
human, mouse, rat
- 保质期:
详见产品包装
- 库存:
充足
- 宿主:
Rabbit
- 应用范围:
WB, IHC, IF/ICC, IF-P, IP, ELISA
- 靶点:
Albumin
- 抗体英文名:
Albumin Polyclonal antibody
- 抗体名:
Albumin Polyclonal antibody
- 规格:
100ul/500ul/1ml
| 规格: | 100ul | 产品价格: | ¥980.0 |
|---|---|---|---|
| 规格: | 500ul | 产品价格: | ¥4000.0 |
| 规格: | 1ml | 产品价格: | ¥6000.0 |
经过测试的应用
| Positive WB detected in | human blood tissue, human plasma, mouse serum, HepG2 cells, mouse liver tissue, rat liver tissue |
| Positive IP detected in | human plasma tissue |
| Positive IHC detected in | human liver tissue, mouse liver tissue, human normal colon Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF-P detected in | mouse liver tissue |
| Positive IF/ICC detected in | L02 cells |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:5000-1:50000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:200-1:2000 |
| Immunofluorescence (IF)-P | IF-P : 1:50-1:500 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:200-1:800 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
16475-1-AP targets Albumin in WB, IHC, IF/ICC, IF-P, IP, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.
| 经测试应用 | WB, IHC, IF/ICC, IF-P, IP, ELISA |
| 文献引用应用 | WB, IHC, IF, CoIP, ELISA |
| 经测试反应性 | human, mouse, rat |
| 文献引用反应性 | human, mouse, rat, goat |
| 免疫原 |
CatNo: Ag9569 Product name: Recombinant human Albumin protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 256-609 aa of BC034023 Sequence: SKLVTDLTKVHTECCHGDLLECADDRADLAKYICENQDSISSKLKECCEKPLLEKSHCIAEVENDEMPADLPSLAADFVESKDVCKNYAEAKDVFLGMFLYEYARRHPDYSVVLLLRLAKTYETTLEKCCAAADPHECYAKVFDEFKPLVEEPQNLIKQNCELFEQLGEYKFQNALLVRYTKKVPQVSTPTLVEVSRNLGKVGSKCCKHPEAKRMPCAEDYLSVVLNQLCVLHEKTPVSDRVTKCCTESLVNRRPCFSALEVDETYVPKEFNAETFTFHADICTLSEKERQIKKQTALVELVKHKPKATKEQLKAVMDDFAAFVEKCCKADDKETCFAEEGKKLVAASQAALGL |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | albumin |
| 别名 | ALB, Serum albumin, HSA |
| 计算分子量 | 609 aa, 69 kDa |
| 观测分子量 | 66 kDa |
| GenBank蛋白编号 | BC034023 |
| 基因名称 | Albumin |
| Gene ID (NCBI) | 213 |
| RRID | AB_2242567 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | P02768 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
Albumin is the most abundant protein in blood plasma. Alterations of level of serum albumin are linked to variety of diseases. Albumin is expressed exclusively by well-differentiated hepatocytes, thus anti-albumin has been used to mark hepatocytes. In additon, glycated serum albumin is also a potential diabetes biomarker. The N-terminal 24aa of albumin will be cleaved to generate the mature form of serum albumin with MW 66 kDa. (21388516, 23832071)
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文献和实验| Species | Application | Title |
|---|---|---|
| human,mouse | WB | Science Enterically derived high-density lipoprotein restrains liver injury through the portal vein.Authors - Yong-Hyun Han |
| mouse | IF | Signal Transduct Target Ther MDIG-mediated H3K9me3 demethylation upregulates Myc by activating OTX2 and facilitates liver regenerationAuthors - Jinpeng Du |
| human,mouse | WB,IF | Signal Transduct Target Ther The AKAP12-PKA axis regulates lipid homeostasis during alcohol-associated liver diseaseAuthors - Chandana Thimme Gowda |
| human, mouse | WB,IF | Nat Immunol Exosomes mediate the cell-to-cell transmission of IFN-α-induced antiviral activity.Authors - Li Jianhua J |
| human | IHC | Immunity The zinc finger protein Miz1 suppresses liver tumorigenesis by restricting hepatocyte-driven macrophage activation and inflammation.Authors - Wenjie Zhang |
| mouse | WB | Nat Cell Biol Manganese regulation of COPII condensation controls circulating lipid homeostasisAuthors - Xiao Wang |
Western Blot Detection of Adrenergic Receptors
for specific antibody–antigen reactions, excess binding sites are blocked by immersing the membrane in a blocking solution containing albumin, nonfat milk, or detergent, such as Tween-20 (6 ). The membrane is probed with an appropriate dilution of the primary
International (Cupertino, CA). BSA-NHS slides, displaying activated amino and carboxyl groups on the surface of an immobilized layer of bovine serum albumin (BSA), were fabricated as follows. 10.24 g N,N'-disuccinimidyl carbonate (100 mM) and 6.96 ml N,N
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