- ￥1350 - 3000
- Proteintech
- 16382-1-AP
- 2025年10月13日
- WB, IHC, IF/ICC, IF-P, FC (Intra), IP, ELISA
- Rabbit
- human, mouse, rat
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- 详细信息
- 文献和实验
- 技术资料
- 免疫原：
Recombinant human Alpha-1-Antitrypsin protein
- 亚型：
IgG
- 保存条件：
Store at -20°C. Stable for one year after shipment.
- 克隆性：
Polyclonal
- 标记物：
Unconjugated
- 适应物种：
human, mouse, rat
- 保质期：
详见产品包装
- 库存：
充足
- 宿主：
Rabbit
- 应用范围：
WB, IHC, IF/ICC, IF-P, FC (Intra), IP, ELISA
- 靶点：
Alpha-1-Antitrypsin
- 抗体英文名：
Alpha-1-Antitrypsin Polyclonal antibody
- 抗体名：
Alpha-1-Antitrypsin Polyclonal antibody
- 规格：
50ul/100ul/150ul
| 规格： | 50ul | 产品价格： | ￥1350.0 |
|---|---|---|---|
| 规格： | 100ul | 产品价格： | ￥2200.0 |
| 规格： | 150ul | 产品价格： | ￥3000.0 |
经过测试的应用
| Positive WB detected in | HeLa cells, human kidney tissue, human placenta tissue, human urine sample, mouse liver tissue, mouse lung tissue, mouse skin tissue, HepG2 cells, mouse kidney tissue, human saliva |
| Positive IP detected in | mouse kidney tissue |
| Positive IHC detected in | mouse liver tissue, human colon tissue, human liver cancer tissue, mouse kidney tissue, rat kidney tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF-P detected in | mouse liver tissue |
| Positive IF/ICC detected in | HepG2 cells |
| Positive FC (Intra) detected in | HepG2 cells |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:5000-1:50000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:400-1:1600 |
| Immunofluorescence (IF)-P | IF-P : 1:50-1:500 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:400-1:1600 |
| Flow Cytometry (FC) (INTRA) | FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
16382-1-AP targets Alpha-1-Antitrypsin in WB, IHC, IF/ICC, IF-P, FC (Intra), IP, ELISA applications and shows reactivity with human, mouse, rat samples.
| 经测试应用 | WB, IHC, IF/ICC, IF-P, FC (Intra), IP, ELISA |
| 文献引用应用 | WB, IHC, IF, IP |
| 经测试反应性 | human, mouse, rat |
| 文献引用反应性 | human, mouse, rat |
| 免疫原 |
CatNo: Ag9369 Product name: Recombinant human Alpha-1-Antitrypsin protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 28-418 aa of BC015642 Sequence: QGDAAQKTDTSHHDQDHPTFNKITPNLAEFAFSLYRQLAHQSNSTNIFFSPVSIATAFAMLSLGTKADTHDEILEGLNFNLTEIPEAQIHEGFQELLRTLNQPDSQLQLTTGNGLFLSEGLKLVDKFLEDVKKLYHSEAFTVNFGDTEEAKKQINDYVEKGTQGKIVDLVKELDRDTVFALVNYIFFKGKWERPFEVKDTEEEDFHVDQATTVKVPMMKRLGMFNIQHCKKLSSWVLLMKYLGNATAIFFLPDEGKLQHLENELTHDIITKFLENEDRRSASLHLPKLSITGTYDLKSVLGQLGITKVFSNGADLSGVTEEAPLKLSKAVHKAVLTIDEKGTEAAGAMFLEAIPMSIPPEVKFNKPFVFLMIEQNTKSPLFMGKVVNPTQK |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | serpin peptidase inhibitor, clade A (alpha-1 antiproteinase, antitrypsin), member 1 |
| 别名 | a1-antitrypsin, Serpin A1, SERPINA1, A1A, A-1-Antitrypsin |
| 计算分子量 | 418 aa, 47 kDa |
| 观测分子量 | 47-51 kDa |
| GenBank蛋白编号 | BC015642 |
| 基因名称 | Alpha 1-Antitrypsin |
| Gene ID (NCBI) | 5265 |
| RRID | AB_10641185 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | P01009 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
SERPINA1 is the gene for a protein called alpha-1-antitrypsin (AAT), which is a serine protease inhibitor whose targets include elastase, plasmin, thrombin, trypsin, chymotrypsin, and plasminogen activator. AAT is a glycoprotein synthesized primarily by hepatocytes, with smaller amountssynthesized by intestinal epithelial cells, neutrophils, pulmonary alveolar cells and macrophages. AAT is the most abundant, endogenous serine protease inhibitor in blood circulation and it has been implicated in regulating vital fluid phase biological events such as blood coagulation, fibrinolysis, complement activation, apoptosis, reproduction, tumor progression and inflammatory response. The primary function of AAT is thought to be the inactivation of neutrophil elastase and other endogenous serine proteases. Defects in SERPINA1 can cause emphysema or liver disease. Alpha 1 Antitrypsin has two bands (PMID: 36703990, PMID: 26869867).
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文献和实验| Species | Application | Title |
|---|---|---|
| human | WB | Cell Neutrophil elastase selectively kills cancer cells and attenuates tumorigenesis.Authors - Chang Cui |
| human | WB | Nat Commun Alpha-1 antitrypsin inhibits TMPRSS2 protease activity and SARS-CoV-2 infection.Authors - Lukas Wettstein |
| mouse | WB | Nat Commun The RNA-binding protein vigilin regulates VLDL secretion through modulation of Apob mRNA translation.Authors - Mehrpouya B Mobin |
| human | IF | Matrix Biol Proteome-wide and matrisome-specific atlas of the human ovary computes fertility biomarker candidates and open the way for precision oncofertility.Authors - Emna Ouni |
| human | IF | Int J Mol Sci Vesicular Integral-Membrane Protein 36 Is Involved in the Selective Secretion of Fucosylated Proteins into Bile Duct-like Structures in HepG2 CellsAuthors - Mizuki Muranaka |
| human | WB | J Nutr Biochem Characterization of dietary and herbal sourced natural compounds that modulate SEL1L-HRD1 ERAD activity and alleviate protein misfolding in the ERAuthors - Jifeng Yang |
accumulation by LPS, as TLR4 can use either MyD88 or TRIF. The net result of these interactions was activation of the transcription factors NF-{kappa}B and AP-1, and synthesis of TNF-{alpha} mRNA. TNF-{alpha} acts through an apparent autocrine pathway
：FITC 羊抗小鼠（绿色）稀释度 1:700 室温下孵育 1 小时。复染色：Hoechst（蓝色）细胞核染色，1:4,000 室温下孵育 1 小时， 细胞定位：路易体内含物；放大倍数：20x。 注射了 1 型小鼠α-突触核蛋白 PFFs（SPR-324）的大鼠脑免疫组织化学分析。实验物种：Sprague-Dawley 大鼠（母），在大鼠脑中有两个注射点，分别是 AP+1.6、ML+2.4、DV-4.2 和 AP-1.4、ML+0.2、DV-2.8，每个注射点注射 2 µL 1 型小鼠 α-突触核蛋白
）、Anti-IgA （alpha）】 在一些特殊的实验里，只需要检测样本里的IgG或者IgM，常规的Anti-IgG （H+L）由于与IgG的重链和轻链都有结合反应，而IgG、IgM、IgA都有保守的轻链结构域，因此不能特异性的检测某种类型的IgG或者IgM。这时候就需要针对特殊类型Ig分子的二抗。***捷备有全面的二抗现货产品并能提供最完整的二抗产品线。主要二抗品牌为全球最大的二抗产品Jackson二抗以及前沿抗体生产商EarthOx。按照标记分，有HRP、AP、生物素等标记二抗，FITC
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