- ￥1350 - 3000
- Proteintech
- 14520-1-AP
- 2025年10月13日
- WB, IHC, IF/ICC, IP, ELISA
- Rabbit
- human, mouse, rat
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- 详细信息
- 文献和实验
- 技术资料
- 免疫原：
Recombinant human UBC12 protein
- 亚型：
IgG
- 保存条件：
Store at -20°C. Stable for one year after shipment.
- 克隆性：
Polyclonal
- 标记物：
Unconjugated
- 适应物种：
human, mouse, rat
- 保质期：
详见产品包装
- 库存：
充足
- 宿主：
Rabbit
- 应用范围：
WB, IHC, IF/ICC, IP, ELISA
- 靶点：
UBC12
- 抗体英文名：
UBC12 Polyclonal antibody
- 抗体名：
UBC12 Polyclonal antibody
- 规格：
50ul/100ul/150ul
| 规格： | 50ul | 产品价格： | ￥1350.0 |
|---|---|---|---|
| 规格： | 100ul | 产品价格： | ￥2200.0 |
| 规格： | 150ul | 产品价格： | ￥3000.0 |
经过测试的应用
| Positive WB detected in | HEK-293T cells, L02 cells, SMMC-7721 cells, HEK-293 cells, Ramos cells |
| Positive IP detected in | L02 cells |
| Positive IHC detected in | mouse ovary tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | L02 cells |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:20-1:200 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
14520-1-AP targets UBC12 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.
| 经测试应用 | WB, IHC, IF/ICC, IP, ELISA |
| 文献引用应用 | WB, IHC, IF |
| 经测试反应性 | human, mouse, rat |
| 文献引用反应性 | human, mouse |
| 免疫原 |
CatNo: Ag5984 Product name: Recombinant human UBC12 protein Source: e coli.-derived, PET28a Tag: 6*His Domain: 1-183 aa of BC058924 Sequence: MIKLFSLKQQKKEEESAGGTKGSSKKASAAQLRIQKDINELNLPKTCDISFSDPDDLLNFKLVICPDEGFYKSGKFVFSFKVGQGYPHDPPKVKCETMVYHPNIDLEGNVCLNILREDWKPVLTINSIIYGLQYLFLEPNPEDPLNKEAAEVLQNNRRLFEQNVQRSMRGGYIGSTYFERCLK |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | ubiquitin-conjugating enzyme E2M (UBC12 homolog, yeast) |
| 别名 | UBE2M, NEDD8-conjugating enzyme Ubc12, NEDD8 protein ligase, NEDD8 conjugating enzyme Ubc12, NEDD8 carrier protein |
| 计算分子量 | 21 kDa |
| 观测分子量 | 21 kDa |
| GenBank蛋白编号 | BC058924 |
| 基因名称 | UBC12 |
| Gene ID (NCBI) | 9040 |
| RRID | AB_2878063 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | P61081 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
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文献和实验| Species | Application | Title |
|---|---|---|
| human | WB,IF | Nat Commun A potent small-molecule inhibitor of the DCN1-UBC12 interaction that selectively blocks cullin 3 neddylation.Authors - Haibin Zhou |
| human | WB | Elife Genome-wide CRISPR screen identifies noncanonical NF-κB signaling as a regulator of density-dependent proliferation.Authors - Maria Fomicheva
|
| human | WB | Cancer Biol Med CUL4 E3 ligase regulates the proliferation and apoptosis of lung squamous cell carcinoma and small cell lung carcinoma.Authors - Ting Li |
| human,mouse | WB,IF | Eur J Pharmacol Camk2n1 deficiency reduces the NaCl cotransporter activity through the CUL3/KLHL3/WNK4 complex in the kidneyAuthors - Ya Zhang |
| human | IHC,WB, IF | Exp Cell Res NPRL2 reduces the niraparib sensitivity of castration-resistant prostate cancer via interacting with UBE2M and enhancing neddylation.Authors - Xin Zhao |
| human | WB | Biochem Biophys Res Commun Disruption of protein neddylation with MLN4924 attenuates paclitaxel-induced apoptosis and microtubule polymerization in ovarian cancer cells.Authors - Xiaoling Hong |
人 apelin 1 2 (AP1 2 ) 酶联免疫 分析（ ELISA ） 试剂 盒使用说明书 本试剂仅供研究使用 目的：本试剂盒用于测定人血清，血浆及相关液体样本中 apelin 12(AP12) 的 含量。 实验原理 ： 本试剂盒应用双抗体夹心法测定 标本 中 人 apelin 1 2 (AP1 2 ) 水平。用纯化的 抗- apelin 1 2 (AP1 2 ) 抗体包被微孔板，制成固相抗体，往包被单抗的微孔中
Regulation of AP-1 Activity by TGF
The activator protein-1 (AP-1) transcriptional complex is composed of DNA binding proteins belonging to the Jun and Fos proto-oncogene families, which play an important role in cell proliferation and transformation (reviewed in ref . 1 ). AP
上海西唐生物科技有限公司 021-55229872, 65333639 www.westang.com 人转录因子AP-1 ( AP-1 )ELISA 试剂盒 ( 用于血清、血浆、细胞培养上清液和其它生物体液内 ) 原理 本实验采用双抗体夹心 ABC-ELISA 法。用抗人 AP-1 单抗包被于酶标板上，标准品和样品中的 AP-1与单抗结合，加入生物素化的抗人 AP
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