- ￥1350 - 3000
- Proteintech
- 14018-1-AP
- 2025年10月13日
- WB, IHC, ELISA
- Rabbit
- human
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- 详细信息
- 询价记录
- 文献和实验
- 技术资料
- 免疫原：
Recombinant human BACH1 protein
- 亚型：
IgG
- 保存条件：
Store at -20°C. Stable for one year after shipment.
- 克隆性：
Polyclonal
- 标记物：
Unconjugated
- 适应物种：
human
- 保质期：
详见产品包装
- 库存：
充足
- 宿主：
Rabbit
- 应用范围：
WB, IHC, ELISA
- 靶点：
BACH1
- 抗体英文名：
BACH1 Polyclonal antibody
- 抗体名：
BACH1 Polyclonal antibody
- 规格：
50ul/100ul/150ul
| 规格： | 50ul | 产品价格： | ￥1350.0 |
|---|---|---|---|
| 规格： | 100ul | 产品价格： | ￥2200.0 |
| 规格： | 150ul | 产品价格： | ￥3000.0 |
经过测试的应用
| Positive WB detected in | HEK-293 cells, MCF-7 cells, Raji cells, HeLa cells, Jurkat cells, K-562 cells |
| Positive IHC detected in | human cervical cancer tissue, human breast hyperplasia tissue, human breast cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:5000-1:50000 |
| Immunohistochemistry (IHC) | IHC : 1:750-1:3000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
14018-1-AP targets BACH1 in WB, IHC, IF, ChIP, ELISA applications and shows reactivity with human samples.
| 经测试应用 | WB, IHC, ELISA |
| 文献引用应用 | WB, IHC, IF, ChIP |
| 经测试反应性 | human |
| 文献引用反应性 | human, mouse, rat |
| 免疫原 |
CatNo: Ag5128 Product name: Recombinant human BACH1 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 387-736 aa of BC063307 Sequence: SSVEREVAEHLAKGFWSDICSTDTPCQMQLSPAVAKDGSEQISQKRSECPWLGIRISESPEPGQRTFTTLSSVNCPFISTLSTEGCSSNLEIGNDDYVSEPQQEPCPYACVISLGDDSETDTEGDSESCSAREQECEVKLPFNAQRIISLSRNDFQSLLKMHKLTPEQLDCIHDIRRRSKNRIAAQRCRKRKLDCIQNLESEIEKLQSEKESLLKERDHILSTLGETKQNLTGLCQKVCKEAALSQEQIQILAKYSAADCPLSFLISEKDKSTPDGELALPSIFSLSDRPPAVLPPCARGNSEPGYARGQESQQMSTATSEQAGPAEQCRQSGGISDFCQQMTDKCTTDE |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | BTB and CNC homology 1, basic leucine zipper transcription factor 1 |
| 别名 | BACH 1, BACH1, BTB and CNC homolog 1, HA2303 |
| 计算分子量 | 82 kDa |
| 观测分子量 | 100-110 kDa |
| GenBank蛋白编号 | BC063307 |
| 基因名称 | BACH1 |
| Gene ID (NCBI) | 571 |
| ENSEMBL Gene ID | ENSG00000156273 |
| RRID | AB_2274498 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | O14867 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
BTB and CNC homology 1 (Bach1) is a transcription factor expressed in many tissues, with key roles in cell cycle
regulation in homeostasis and in cancer in disease.
What is the molecular weight of BACH1?
Bach1 consists of 736 amino acids and is a member of the cap'n'collar type of basic region leucine zipper factor
family (CNC-bZip) with several atypical protein domains. Although its predicted MW is 82 kDa, it runs
anomalously at 100-110 kDa in SDS-PAGE.
What is the cellular localization of BACH1?
As it is a transcription factor, BACH1 is found in the nucleus of a number of mammalian cells, where it forms a
heterodimer with small Maf proteins. Once it has formed part of a heterodimer, BACH1 is able to bind to the
Maf recognition elements (MAREs) of target genes. During oxidative stress, BACH1 can be inactivated either by
heme or by cadmium, at which point it is exported from the nucleus into the cytoplasm, where it localizes to
microtubules (PMID: 15809329).
What is the function of BACH1?
BACH1 regulates the production of reactive oxygen species, with many of its downstream targets such as heme
oxygenase-1 (HO-1) and NADPH quinone oxidoreductase 1 (NQO1) (PMID: 12356737; PMID: 15734732) involved
in the oxidative stress response. When the cell undergoes oxidative stress, BACH1 is displaced by nuclear factor
(erythroid-derived2)-like-2 (Nrf2), as both compete in binding to the MAREs in target genes. BACH1 also plays a
role in cell proliferation and survival, as it regulates several cell cycle genes such as cyclin-dependent kinase 6
(CDK6) and BCL2-like 11 (BCL2L11).
What is the role of BACH1 in disease?
BACH1 has been described in a number of cancer types, including breast, prostate, and melanoma. The function
of BACH1 appears varied in these tissues, with evidence that it may both promote and repress metastasis,
influence epigenetics, and regulate the growth of cancer cells (PMID: 22875853; PMID: 28889753; PMID: 26787
892). BACH1 may act on the same gene in different contexts to repress or activate it (PMID: 8887638).
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- 作者
- 内容
- 询问日期
文献和实验| Species | Application | Title |
|---|---|---|
| human | WB,IHC,IF | Sci Transl Med PTEN status determines chemosensitivity to proteasome inhibition in cholangiocarcinoma.Authors - Tian-Yi Jiang |
| human | WB | Autophagy MEF2D-NR4A1-FAM134B2-mediated reticulophagy contributes to amino acid homeostasisAuthors - Yuji Shiozaki |
| human | WB | J Exp Clin Cancer Res Lnc AC016727.1/BACH1/HIF-1 α signal loop promotes the progression of non-small cell lung cancerAuthors - Li Zhang |
| human,mouse | WB,IHC,IF | J Nanobiotechnology Eriodictyol-cisplatin coated nanomedicine synergistically promote osteosarcoma cells ferroptosis and chemosensitivityAuthors - Zili Lin |
| human | WB,IF,IHC | Cancer Lett PTEN deficiency facilitates the therapeutic vulnerability to proteasome inhibitor bortezomib in gallbladder cancer.Authors - Tian-Yi Jiang |
| human | WB | iScience Regulation of DNA damage response by trimeric G-proteinsAuthors - Amer Ali Abd El-Hafeez |
因此，综合来说，EarthOx的GST标签抗体 （货号#E022040）是一款同时具有高特异性、高灵敏度、多应用类型以及性价比的GST标签抗体。同时，我们也提供其他品牌的GST标签抗体，如Proteintech的GST多克隆抗体 ――GST PolyClonal Antibody（货号#10000-0-AP）以及Abcam、Santa等多种GST标签抗体产品。更多GST标签抗体以及其他如His、HA、Myc、GFP、Flag等其他标签抗体，请电话垂询。美国EarthOx品牌是一家提供高性价比免疫学和细胞
实验，请根据说明书列出已验证的种属来选择适合你实验的抗体。 （4）样本蛋白的结构性质。了解样本蛋白的结构性质有助于选择最合适的抗体，待测样本蛋白的结构域和样本在提取和处理过程中是否会变性，蛋白空间构象的改变，会影响抗体的免疫亲和反应。 （5）单多克隆抗体的选择。市面上的抗体还是以多克隆抗体为主，一般单克隆抗体特异性强，但亲和力相对小，检测抗原灵敏度相对就低；而多克隆抗体特异性稍弱，但抗体的亲和力强，灵敏度高，但易出现非特异性染色（可以通过封闭等避免
发生，最好在特异性抗体处理切片之前，选择与二抗种属相同的非免疫血清封闭电荷，阻止一抗与之结合，抑制非特异性背景着色。常用方法是用2%～10%羊血清或2%～5%牛血清白蛋白在室温下作用10~30分钟。但应注意此种结合不牢固，所以最好不要冲洗，倾去余液直接加一抗。SABC或SP免疫组织化学试剂盒中常配有非免疫血清。 7．第一抗体孵育 滴加特异性一抗于切片上，4℃孵育24～48小时，或室温孵育过夜，也可在37~C孵育1～2小时。之后，用PBS冲洗3次，每次5分钟。一抗分为多克隆抗体和单克
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