- ￥1350 - 3000
- Proteintech
- 12555-1-AP
- 2026年05月21日
- WB, IHC, IP, ELISA
- Rabbit
- human, mouse, rat
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- 详细信息
- 文献和实验
- 技术资料
- 免疫原：
Recombinant human PrP protein
- 亚型：
IgG
- 保存条件：
Store at -20°C. Stable for one year after shipment.
- 克隆性：
Polyclonal
- 标记物：
Unconjugated
- 适应物种：
human, mouse, rat
- 保质期：
详见产品包装
- 库存：
充足
- 宿主：
Rabbit
- 应用范围：
WB, IHC, IP, ELISA
- 靶点：
Prion protein PrP/CD230
- 抗体英文名：
Prion protein PrP/CD230 Polyclonal antibody
- 抗体名：
Prion protein PrP/CD230 Polyclonal antibody
- 规格：
50ul/100ul/150ul
| 规格： | 50ul | 产品价格： | ￥1350.0 |
|---|---|---|---|
| 规格： | 100ul | 产品价格： | ￥2200.0 |
| 规格： | 150ul | 产品价格： | ￥3000.0 |
经过测试的应用
| Positive WB detected in | mouse brain tissue, human brain tissue, rat brain tissue |
| Positive IP detected in | mouse brain tissue |
| Positive IHC detected in | human gliomas tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:20-1:200 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
12555-1-AP targets Prion protein PrP/CD230 in WB, IHC, IP, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.
| 经测试应用 | WB, IHC, IP, ELISA |
| 文献引用应用 | WB, IHC, IP, CoIP |
| 经测试反应性 | human, mouse, rat |
| 文献引用反应性 | human, mouse, rat |
| 免疫原 |
CatNo: Ag3257 Product name: Recombinant human PrP protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 17-240 aa of BC022532 Sequence: SDLGLCKKRPKPGGWNTGGSRYPGQGSPGGNRYPPQGGGGWGQPHGGGWGQPHGGGWGQPHGGGWGQPHGGGWGQGGGTHSQWNKPSKPKTNMKHMAGAAAAGAVVGGLGGYVLGSAMSRPIIHFGSDYEDRYYRENMHRYPNQVYYRPMDEYSNQNNFVHDCVNITIKQHTVTTTTKGENFTETDVKMMERVVEQMCITQYERESQAYYKRGSSMVLFSSPPV |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | prion protein |
| 别名 | Prion protein PrP, PRNP, PrP, Alternative prion protein, AltPrP |
| 计算分子量 | 34 kDa |
| 观测分子量 | 30 kDa |
| GenBank蛋白编号 | BC022532 |
| 基因名称 | PrP |
| Gene ID (NCBI) | 5621 |
| RRID | AB_2237745 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | P04156 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
Prion protein (PRNP) is a ubiquitous membrane glycoprotein whose abnormal self-replicating, misfolded form is widely believed to cause several central nervous system disorders, collectively known as Transmissible Spongiform Encephalopathies (TSE). Prion diseases are TSEs, attributed to conformational conversion of the cellular prion protein (PrPC) into an abnormal conformer that accumulates in the brain. The two isoforms, PrPC and PrPS, have the same primary amino acid sequence and only differ in conformation. While PrPC is composed of 42% α-helix and only 3% β-sheet, PrPSc is composed of 30% α-helix and 43% β-sheet. PrPC converts to its pathogenic isoform when the region corresponding to the residues 108-144 fold into β-sheets. PrPC is very soluble in detergents and easily digested by proteases while the PrPSc is insoluble in detergents and resistant to protease digestion. Prion diseases exist in infectious, sporadic, and genetic forms.
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文献和实验| Species | Application | Title |
|---|---|---|
| mouse,human | WB,IP | Neuron Muskelin Coordinates PrPC Lysosome versus Exosome Targeting and Impacts Prion Disease Progression.Authors - Frank F Heisler |
| rat | WB | PLoS Biol TMED9 coordinates the clearance of misfolded GPI-anchored proteins out of the ER and into the GolgiAuthors - Elsa Ronzier |
| human | IHC | Front Mol Biosci Single-cell transcriptomics uncover the key ferroptosis regulators contribute to cancer progression in head and neck squamous cell carcinomaAuthors - Fei Liu |
| human | WB | PLoS One Copper homeostasis-associated gene PRNP regulates ferroptosis and immune infiltration in breast cancerAuthors - Changwei Lin |
| human | WB,IHC,CoIP | Mol Carcinog Inhibition of the RBMS1/PRNP axis improves ferroptosis resistance-mediated oxaliplatin chemoresistance in colorectal cancerAuthors - Yini Xu |
| human | WB | Front Cell Dev Biol PRNP is a pan-cancer prognostic and immunity-related to EMT in colorectal cancerAuthors - Haifeng Chen |
[资源] 所有的看家基因(housekeeping genes)列表+引物设计服务
1, mRNA 8138 NM_004689 Homo sapiens metastasis associated 1 (MTA1), mRNA 506 NM_001848 Homo sapiens collagen, type VI, alpha 1 (COL6A1), mRNA 757 NM_004068 Homo sapiens adaptor-related protein complex 2, mu 1 subunit (AP2M1), mRNA 2188 NM
【资源】全面归类网址：毕赤酵母表达各种来源蛋白（细菌，真菌，植物，人类等）
a-hydroxylase/C17,20-lyaseI[194]Syrian golden hamster prion protein PrPcI,[195]Tricosurus vulpecula (possum) cytokine stem cell factor (SCF)S, α-MF, 45 mg/L501Humansa(1,3/4) fucosyltransferaseS, 30 mg/L, α-MF[130]a-1,2-mannosidase 1B w/o TM domainS, α-MF[196]a-N
Antitermination protein (抗终止蛋白质)：能够使RNA聚合酶通过一定的终止位点的蛋白质质。 AP endonucleases (AP 核酸内切酶)：剪切掉DNA 5'端脱嘌呤和脱嘧啶位点的酶 Apoptosis (细胞凋亡)：细胞进行程序性死亡的能力；对刺激应答使通过一系列特定反应摧毁细胞的途径发生。 Archeae (古细菌)：进化中与原核和真核不同的一个分支。 Ascue (子囊)：真菌的子囊包含四个或八个(单一的)孢子，表示一次减数分裂的产物。 Att sites (Att
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