- ￥1350 - 3000
- Proteintech
- 12143-1-AP
- 2026年05月21日
- WB, IHC, IF/ICC, IF-P, FC (Intra), IP, ELISA
- Rabbit
- human, mouse, rat
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- 详细信息
- 文献和实验
- 技术资料
- 免疫原：
Recombinant human TP63 protein
- 亚型：
IgG
- 保存条件：
Store at -20°C. Stable for one year after shipment.
- 克隆性：
Polyclonal
- 标记物：
Unconjugated
- 适应物种：
human, mouse, rat
- 保质期：
详见产品包装
- 库存：
充足
- 宿主：
Rabbit
- 应用范围：
WB, IHC, IF/ICC, IF-P, FC (Intra), IP, ELISA
- 靶点：
p63
- 抗体英文名：
p63 Polyclonal antibody
- 抗体名：
p63 Polyclonal antibody
- 规格：
50ul/100ul/150ul
| 规格： | 50ul | 产品价格： | ￥1350.0 |
|---|---|---|---|
| 规格： | 100ul | 产品价格： | ￥2200.0 |
| 规格： | 150ul | 产品价格： | ￥3000.0 |
经过测试的应用
| Positive WB detected in | A431 cells, human heart tissue, mouse heart tissue, U-937 cells, Apoptosised HeLa cells, HaCaT cells, PC-12 cells, mouse thymus tissue |
| Positive IP detected in | A431 cells |
| Positive IHC detected in | human tonsillitis tissue, human bowen disease, human lung squamous cell carcinoma tissue, human prostate cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF-P detected in | human lung cancer tissue |
| Positive IF/ICC detected in | A431 cells |
| Positive FC (Intra) detected in | A431 cells |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:500-1:3000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:200-1:800 |
| Immunofluorescence (IF)-P | IF-P : 1:50-1:500 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:500-1:2000 |
| Flow Cytometry (FC) (INTRA) | FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
12143-1-AP targets p63 in WB, IHC, IF/ICC, IF-P, FC (Intra), IP, CoIP, ChIP, ELISA applications and shows reactivity with human, mouse, rat samples.
| 经测试应用 | WB, IHC, IF/ICC, IF-P, FC (Intra), IP, ELISA |
| 文献引用应用 | WB, IHC, IF, IP, CoIP, ChIP |
| 经测试反应性 | human, mouse, rat |
| 文献引用反应性 | human, mouse, rat |
| 免疫原 |
CatNo: Ag2791 Product name: Recombinant human TP63 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 381-680 aa of BC039815 Sequence: NTHGIQMTSIKKRRSPDDELLYLPVRGRETYEMLLKIKESLELMQYLPQHTIETYRQQQQQQHQHLLQKQTSIQSPSSYGNSSPPLNKMNSMNKLPSVSQLINPQQRNALTPTTIPDGMGANIPMMGTHMPMAGDMNGLSPTQALPPPLSMPSTSHCTPPPPYPTDCSIVSFLARLGCSSCLDYFTTQGLTTIYQIEHYSMDDLASLKIPEQFRHAIWKGILDHRQLHEFSSPSHLLRTPSSASTVSVGSSETRGERVIDAVRFTLRQTISFPPRDEWNDFNFDMDARRNKQQRIKEEGE |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | tumor protein p63 |
| 别名 | TP63, CUSP, TP 63, Transformation-related protein 63, Tumor protein 63 |
| 计算分子量 | 680 aa, 77 kDa |
| 观测分子量 | 63-68 kDa |
| GenBank蛋白编号 | BC039815 |
| 基因名称 | TP63 |
| Gene ID (NCBI) | 8626 |
| RRID | AB_10597397 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | Q9H3D4 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
TP63, also named KET, P63, P73H, P73L and TP73L, belongs to the p53 family. It is a homologue of the tumor suppressor p53 and p73 genes. It is involved in malignancy acquisition and maintenance of cells. Unlike p53, the p63 gene encodes multiple isotypes with remarkably divergent abilities to transactivate p53 reporter genes and induce apoptosis. TP63 acts as a sequence specific DNA binding transcriptional activator or repressor. TP63 has 12 isoforms with MW 40kd(P40), 50kd(P60),63kd(P63) and 73kd(P73L).
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文献和实验| Species | Application | Title |
|---|---|---|
| mouse | IHC | Nature Divergent transcriptional regulation of astrocyte reactivity across disorders.Authors - Joshua E Burda |
| human | WB,IP,ChIP | Nucleic Acids Res Activation of bivalent factor DLX5 cooperates with master regulator TP63 to promote squamous cell carcinoma.Authors - Yongsheng Huang
|
| human | IF | Cell Rep A Wnt-induced lncRNA-DGCR5 splicing switch drives tumor-promoting inflammation in esophageal squamous cell carcinomaAuthors - Yue Li |
| mouse | IHC | Oncogene TP53, CDKN2A/P16, and NFE2L2/NRF2 regulate the incidence of pure- and combined-small cell lung cancer in mice.Authors - Samera H Hamad |
| human | IF | Elife Chloride channels regulate differentiation and barrier functions of the mammalian airway.Authors - Mu He |
| human | IF | Stem Cell Res Ther Mesenchymal stromal cells promote the formation of lung cancer organoids via Kindlin-2Authors - Zhilin Sui |
因此，综合来说，EarthOx的GST标签抗体 （货号#E022040）是一款同时具有高特异性、高灵敏度、多应用类型以及性价比的GST标签抗体。同时，我们也提供其他品牌的GST标签抗体，如Proteintech的GST多克隆抗体 ――GST PolyClonal Antibody（货号#10000-0-AP）以及Abcam、Santa等多种GST标签抗体产品。更多GST标签抗体以及其他如His、HA、Myc、GFP、Flag等其他标签抗体，请电话垂询。美国EarthOx品牌是一家提供高性价比免疫学和细胞
实验，请根据说明书列出已验证的种属来选择适合你实验的抗体。 （4）样本蛋白的结构性质。了解样本蛋白的结构性质有助于选择最合适的抗体，待测样本蛋白的结构域和样本在提取和处理过程中是否会变性，蛋白空间构象的改变，会影响抗体的免疫亲和反应。 （5）单多克隆抗体的选择。市面上的抗体还是以多克隆抗体为主，一般单克隆抗体特异性强，但亲和力相对小，检测抗原灵敏度相对就低；而多克隆抗体特异性稍弱，但抗体的亲和力强，灵敏度高，但易出现非特异性染色（可以通过封闭等避免
发生，最好在特异性抗体处理切片之前，选择与二抗种属相同的非免疫血清封闭电荷，阻止一抗与之结合，抑制非特异性背景着色。常用方法是用2%～10%羊血清或2%～5%牛血清白蛋白在室温下作用10~30分钟。但应注意此种结合不牢固，所以最好不要冲洗，倾去余液直接加一抗。SABC或SP免疫组织化学试剂盒中常配有非免疫血清。 7．第一抗体孵育 滴加特异性一抗于切片上，4℃孵育24～48小时，或室温孵育过夜，也可在37~C孵育1～2小时。之后，用PBS冲洗3次，每次5分钟。一抗分为多克隆抗体和单克
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