- ￥1350 - 3000
- Proteintech
- 11615-1-AP
- 2025年10月13日
- IHC, ELISA
- Rabbit
- human
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- 详细信息
- 文献和实验
- 技术资料
- 免疫原：
Recombinant human CGB,hCG protein
- 亚型：
IgG
- 保存条件：
Store at -20°C. Stable for one year after shipment.
- 克隆性：
Polyclonal
- 标记物：
Unconjugated
- 适应物种：
human
- 保质期：
详见产品包装
- 库存：
充足
- 宿主：
Rabbit
- 应用范围：
IHC, ELISA
- 靶点：
hCG Beta
- 抗体英文名：
hCG Beta Polyclonal antibody
- 抗体名：
hCG Beta Polyclonal antibody
- 规格：
50ul/100ul/150ul
| 规格： | 50ul | 产品价格： | ￥1350.0 |
|---|---|---|---|
| 规格： | 100ul | 产品价格： | ￥2200.0 |
| 规格： | 150ul | 产品价格： | ￥3000.0 |
经过测试的应用
| Positive IHC detected in | human placenta tissue, human breast cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
11615-1-AP targets hCG Beta in WB, IHC, IF, ELISA applications and shows reactivity with human samples.
| 经测试应用 | IHC, ELISA |
| 文献引用应用 | WB, IHC, IF |
| 经测试反应性 | human |
| 文献引用反应性 | human |
| 免疫原 |
CatNo: Ag2191 Product name: Recombinant human CGB,hCG protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-165 aa of BC022796 Sequence: MEMFQGLLLLLLLSMGGTWASKEPLRPRCRPINATLAVEKEGCPVCITVNTTICAGYCPTMTRVLQGVLPALPQVVCNYRDVRFESIRLPGCPRGVNPVVSYAVALSCQCALCRRSTTDCGGPKDHPLTCDDPRFQDSSSSKAPPPSLPSPSRLPGPSDTPILPQ |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | CG beta |
| 别名 | CGB, CGB,hCG, CG beta, CGB3, CGbeta |
| 计算分子量 | 165 aa, 18 kDa |
| GenBank蛋白编号 | BC022796 |
| 基因名称 | hCG Beta |
| Gene ID (NCBI) | 1082 |
| RRID | AB_2877783 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | P0DN86 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
Beta-hCG (beta-human chorionic gonadotropin) is a glycoprotein hormone produced by the placenta during pregnancy. It is a critical marker used in both obstetrics and oncology for various diagnostic and monitoring purposes. hCG is composed of two subunits: an alpha subunit (similar to other glycoprotein hormones like LH, FSH, and TSH) and a unique beta subunit (β-hCG). The beta subunit is the primary focus for diagnostic tests due to its specificity. β-hCG is initially secreted by the syncytiotrophoblast cells surrounding the developing embryo and later by the placenta.
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文献和实验| Species | Application | Title |
|---|---|---|
| human | WB | J Transl Med STK40 inhibits trophoblast fusion by mediating COP1 ubiquitination to degrade P57Kip2Authors - Xia Li |
| human | WB,IHC | iScience Demethylase FTO-mediated m6A modification of SIK1 modulates placental cytotrophoblast syncytialization in type 2 diabetes mellitusAuthors - Jie Ning |
| human | IF | EBioMedicine Molecular mechanisms underlying altered neurobehavioural development of female offspring of mothers with polycystic ovary syndrome: FOS-mediated regulation of neurotrophins in placenta.Authors - Fangfang Wang |
| human | IF | Cell Signal Hypermethylation and low expression of FOXM1 predisposes women to unexplained recurrent miscarriage by impairing trophoblast stem cell proliferationAuthors - Youqing Jiang |
| human | WB | Mol Cell Endocrinol Activation of G protein-coupled estrogen receptor stimulates placental human chorionic gonadotropin expression through PKA-CREB signalingAuthors - Sizhu Yang |
| human | WB | Biomolecules Calnexin Is Involved in Forskolin-Induced Syncytialization in Cytotrophoblast Model BeWo CellsAuthors - Hitomi Matsukawa |
Human chorionic gonadotropin (hCG) has been considered as a primary target molecule for a contraceptive vaccine by the World Health Organization because of its physiological and temporal specificity. hCG is an essential factor
Transforming Growth Factor Beta: A Plasma Tumor Marker
are prostate-specific antigen (PSA), carcinoembryonic antigen (CEA), β-human chorionic gonadotropin (β-HCG), and CA-125. Tumor markers may be specific for certain tumors, such as PSA for prostate cancer, or more generally expressed, such as CEA.
2. 抗原与填料的连接 这一步是整个纯化过程中最关键的一步，如果在这一步上有什么失误，建议你从step one重新开始吧。 首先要注意一点：常见的连接方法都基于抗原上的游离氨基或者巯基与活化后的beads的连接，因此体系中不应该有其它含有氨基或巯基的化合物，包括尿素、硫脲、盐酸胍、Tris、铵根离子、Beta-巯基乙醇等，如果有这些物质在体系中，应该首先设法除去。 溴化氰活化的琼脂糖与氨基化合物反应的机理如下： 环氧氯丙烷活化的琼脂糖与氨基化合物反应
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