- ￥1350 - 3000
- Proteintech
- 10988-1-AP
- 2025年10月13日
- WB, IP, ELISA
- Rabbit
- human
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- 详细信息
- 文献和实验
- 技术资料
- 免疫原：
Recombinant human BID protein
- 亚型：
IgG
- 保存条件：
Store at -20°C. Stable for one year after shipment.
- 克隆性：
Polyclonal
- 标记物：
Unconjugated
- 适应物种：
human
- 保质期：
详见产品包装
- 库存：
充足
- 宿主：
Rabbit
- 应用范围：
WB, IP, ELISA
- 靶点：
BID
- 抗体英文名：
BID Polyclonal antibody
- 抗体名：
BID Polyclonal antibody
- 规格：
50ul/100ul/150ul
| 规格： | 50ul | 产品价格： | ￥1350.0 |
|---|---|---|---|
| 规格： | 100ul | 产品价格： | ￥2200.0 |
| 规格： | 150ul | 产品价格： | ￥3000.0 |
经过测试的应用
| Positive WB detected in | A431 cells, human brain tissue, Tunicamycin treated HeLa cells, A549 cells, HEK-293 cells, HepG2 cells, Jurkat cells |
| Positive IP detected in | HeLa cells |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:4000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
10988-1-AP targets BID in WB, IHC, IF, IP, ELISA applications and shows reactivity with human samples.
| 经测试应用 | WB, IP, ELISA |
| 文献引用应用 | WB, IHC, IF |
| 经测试反应性 | human |
| 文献引用反应性 | human, mouse, rat, pig |
| 免疫原 |
CatNo: Ag1445 Product name: Recombinant human BID protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-195 aa of BC009197 Sequence: MDCEVNNGSSLRDECITNLLVFGFLQSCSDNSFRRELDALGHELPVLAPQWEGYDELQTDGNRSSHSRLGRIEADSESQEDIIRNIARHLAQVGDSMDRSIPPGLVNGLALQLRNTSRSEEDRNRDLATALEQLLQAYPRDMEKEKTMLVLALLLAKKVASHTPSLLRDVFHTTVNFINQNLRTYVRSLARNGMD |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | BH3 interacting domain death agonist |
| 别名 | BID, FP497, p11 BID, p22 BID |
| 计算分子量 | 22 kDa |
| 观测分子量 | 22 kDa |
| GenBank蛋白编号 | BC009197 |
| 基因名称 | BID |
| Gene ID (NCBI) | 637 |
| RRID | AB_2065624 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | P55957 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
BID, also named as p22 BID, can be cleaved into p11 BID, p13 BID and p15 BID. It is pro-apoptotic molecules. The major proteolytic product p15 BID allows the release of cytochrome c. BID-L, BID-EL and BID-ES induce ICE-like proteases and apoptosis. BID-S does not induce apoptosis. BID counters the protective effect of Bcl-2. (PMID:14583606)
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文献和实验| Species | Application | Title |
|---|---|---|
| rat | WB | Bioact Mater A ROS-responsive, self-immolative and self-reporting hydrogen sulfide donor with multiple biological activities for the treatment of myocardial infarction.Authors - Mengyun Yao |
| mouse | WB | Environ Pollut Silica nanoparticles inducing the apoptosis via microRNA-450b-3p targeting MTCH2 in mice and spermatocyte cell.Authors - Guiqing Zhou |
| rat | WB | Cell Mol Life Sci Clostridioides difficile toxin B alone and with pro-inflammatory cytokines induces apoptosis in enteric glial cells by activating three different signalling pathways mediated by caspases, calpains and cathepsin B.Authors - Katia Fettucciari |
| human | WB | Int J Cancer Tetrandrine induces apoptosis by activating reactive oxygen species and repressing Akt activity in human hepatocellular carcinoma.Authors - Liu Chaoyang C |
| human | WB | Free Radic Biol Med Shikonin, a Chinese plant-derived naphthoquinone, induces apoptosis in hepatocellular carcinoma cells through reactive oxygen species: A potential new treatment for hepatocellular carcinoma.Authors - Gong Ke K |
| rat | WB | Stem Cell Res Ther Adipose-derived stem cells alleviate radiation-induced dermatitis by suppressing apoptosis and downregulating cathepsin F expression.Authors - Chaoling Yao |
=6094439&sty=1&tpg=21&age=0谁用过R&D公司的HRP酶标的羊抗鼠IgG试剂盒http://www.dxy.cn/bbs/post/view?bid=68&id=6009081&sty=1&tpg=21&age=0急需有关酶联反应方面的资料http://www.dxy.cn/bbs/post/view?bid=68&id=6113280&sty=1&tpg=20&age=0AP＋BCIP/NBT 会形成不溶性蓝色物质http://www.dxy.cn/bbs/post/view?bid
/post/view?bid=68&id=6949937&sty=1&tpg=1&age=0 请教 ：GST融合蛋白多克隆抗体的纯化？ http://www.dxy.cn/bbs/post/view?bid=68&id=6950145&sty=1&tpg=1&age=0 【求助】免疫组化和原位杂交 http://www.dxy.cn/bbs/post/view?bid=68&id=6950521&sty=1&tpg=1&age=0 【求助】抗原修复 http://www.dxy.cn/bbs
的影响。5.加抗体标本(和二抗):注意该换枪头时换枪头.标本稀释一般可用pbs稀释,也可用封闭液去稀释.如需加二抗,还要注意二抗的工作浓度,太高浪费,太低则着色浅.6.显色:显色系统又很多,我们一开始做的时候,要选择适宜的显色系统.注意显色系统酶活性底物的保存HRP结合物加硫柳泵，AP结合物可加叠氮钠.7.每次做尽量要把阴阳空三个对照做好,如出现问题也好分析.终于打完了,也到了吃午饭时间了,呵呵.回看一下,写得有点虎头蛇尾了,请各位多多海涵啊,以后有问题可以多多再园子里探讨探讨.我写的也可能
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