- ￥1350 - 3000
- Proteintech
- 10486-1-AP
- 2025年10月13日
- WB, IHC, IF/ICC, IP, ELISA
- Rabbit
- human, mouse, rat
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- 详细信息
- 文献和实验
- 技术资料
- 免疫原：
Recombinant human JUNB protein
- 亚型：
IgG
- 保存条件：
Store at -20°C. Stable for one year after shipment.
- 克隆性：
Polyclonal
- 标记物：
Unconjugated
- 适应物种：
human, mouse, rat
- 保质期：
详见产品包装
- 库存：
充足
- 宿主：
Rabbit
- 应用范围：
WB, IHC, IF/ICC, IP, ELISA
- 靶点：
JUNB
- 抗体英文名：
JUNB Polyclonal antibody
- 抗体名：
JUNB Polyclonal antibody
- 规格：
50ul/100ul/150ul
| 规格： | 50ul | 产品价格： | ￥1350.0 |
|---|---|---|---|
| 规格： | 100ul | 产品价格： | ￥2200.0 |
| 规格： | 150ul | 产品价格： | ￥3000.0 |
经过测试的应用
| Positive WB detected in | HT-1080 cells, HeLa cells, MCF-7 cells, MDA-MB-231 cells |
| Positive IP detected in | HeLa cells |
| Positive IHC detected in | human lymphoma tissue, human breast cancer tissue, human ovary tissue, human testis tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | A431 cells, HeLa cells |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:8000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:500-1:2000 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:400-1:1600 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
10486-1-AP targets JUNB in WB, IHC, IF/ICC, IP, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.
| 经测试应用 | WB, IHC, IF/ICC, IP, ELISA |
| 文献引用应用 | WB, IHC, IF, CoIP |
| 经测试反应性 | human, mouse, rat |
| 文献引用反应性 | human, mouse, rat |
| 免疫原 |
CatNo: Ag0752 Product name: Recombinant human JUNB protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-200 aa of BC004250 Sequence: MCTKMEQPFYHDDSYTATGYGRAPGGLSLHDYKLLKPSLAVNLADPYRSLKAPGARGPGPEGGGGGSYFSGQGSDTGASLKLASSELERLIVPNSNGVITTTPTPPGQYFYPRGGGSGGGAGGAGGGVTEEQEGFADGFVKALDDLHKMNHVTPPNVSLGATGGPPAGPGGVYAGPEPPPVYTNLSSYSPASASSGGAGA |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | jun B proto-oncogene |
| 别名 | AP 1, AP-1 |
| 计算分子量 | 36 kDa |
| 观测分子量 | 38 kDa, 42 kDa |
| GenBank蛋白编号 | BC004250 |
| 基因名称 | JUNB |
| Gene ID (NCBI) | 3726 |
| RRID | AB_2129996 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | P17275 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
JunB is one of the components of the Activator Protein-1 (AP-1) transcription complex that have been implicated in the control ofthe G0/G1 transtion in fibroblasts. AP-1 is a collection of dimers formed by members of the Jun-, Fos-, ATF- and Maf multigene families that bind to specific DNA regulatory elements called AP-1/12-O-tetradecanoylphorbol-13-acetate-responsive elements (TREs) and cAMP-responsive elements (CREs). JunB binds to the DNA sequence 5'-TGA[CG]TCA-3', and involves in the regulation of gene activity following the primary growth factor response. It also acts either as a tumor suppressor or as an oncogene depending on the cell and physiopathological context
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文献和实验| Species | Application | Title |
|---|---|---|
| mouse | WB | Signal Transduct Target Ther MDIG-mediated H3K9me3 demethylation upregulates Myc by activating OTX2 and facilitates liver regenerationAuthors - Jinpeng Du |
| mouse | WB | J Exp Med USP38 critically promotes asthmatic pathogenesis by stabilizing JunB protein.Authors - Siyuan Chen |
| rat,mouse | WB | Pharmacol Res Cyclo-(Phe-Tyr) as a novel cyclic dipeptide compound alleviates ischemic/reperfusion brain injury via JUNB/JNK/NF-κB and SOX5/PI3K/AKT pathways.Authors - Jiale Cai |
| human | WB | Am J Cancer Res PMID: 31285951Authors - Wen-Si Zhao |
| human | WB | J Proteome Res Systematic Survey of the Regulatory Networks of the Long Noncoding RNA BANCR in Cervical Cancer Cells.Authors - Bing Wang |
Oncol Lett Cigarette smoke extract-induced proliferation of normal human urothelial cells via the MAPK/AP-1 pathway.Authors - Hao Geng |
稳定性和半衰期的调节。c―jun73位丝氨酸经JNK磷酸化后可有效地免受泛素化作用，导致c―jun半衰期延长。在非应激细胞中，JNK有促进靶基因产物泛素化的作用，这种作用取决于JNK与c―jun紧密结合的能力，而不是激酶活性。JNK调节c―jun的泛素化依赖于JNK与c―jun的相互结合作用以及c―jun的磷酸化水平。除c―jun是JNKMAPK的底物外，JNKMAPK也可磷酸化并激活其他转录因子。激活的JNK可使AP―1复合体中的其他组分蛋白发生磷酸化，包括junB、junD、ATF2和Elk
C. David/赵玉沛院士/吴文铭揭示「癌王发迹史」，为早期诊断与治疗提供潜在的靶点和思路
高表达的转录因子的筛选。研究发现，敲除掉 AP1 家族中的 Fosl1，Junb，Klf 家族中的 Klf5 与 Fox 家族中的 Foxa2，都对胰腺癌的生存产生了阻碍（图三，a）。为了探究 Junb，Fosl1，Klf5 与 Foxa2 在胰腺癌的发展早期的作用，作者利用腺相关病毒血清型（携带 Cdkn2a, Trp53, 和 Smad4 这三个在胰腺癌中高突变的肿瘤抑制基因的 sgRNA 与目标转录因子的 sgRNA）去分别敲除掉这几个转录因子。跟对照组的 PDAC 相比，敲除掉 Junb
因此，综合来说，EarthOx的GST标签抗体 （货号#E022040）是一款同时具有高特异性、高灵敏度、多应用类型以及性价比的GST标签抗体。同时，我们也提供其他品牌的GST标签抗体，如Proteintech的GST多克隆抗体 ――GST PolyClonal Antibody（货号#10000-0-AP）以及Abcam、Santa等多种GST标签抗体产品。更多GST标签抗体以及其他如His、HA、Myc、GFP、Flag等其他标签抗体，请电话垂询。美国EarthOx品牌是一家提供高性价比免疫学和细胞
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