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- 详细信息
- 询价记录
- 文献和实验
- 技术资料
- 免疫原:
Recombinant human CDC42 protein
- 亚型:
IgG
- 保存条件:
Store at -20°C. Stable for one year after shipment.
- 克隆性:
Polyclonal
- 标记物:
Unconjugated
- 适应物种:
human, mouse, rat
- 保质期:
详见产品包装
- 库存:
充足
- 宿主:
Rabbit
- 应用范围:
WB, IHC, IF/ICC, ELISA
- 靶点:
CDC42
- 抗体英文名:
CDC42 Polyclonal antibody
- 抗体名:
CDC42 Polyclonal antibody
- 规格:
50ul/100ul/150ul
| 规格: | 50ul | 产品价格: | ¥1350.0 |
|---|---|---|---|
| 规格: | 100ul | 产品价格: | ¥2200.0 |
| 规格: | 150ul | 产品价格: | ¥3000.0 |
经过测试的应用
| Positive WB detected in | HT-29 cells, C6 cells, HeLa cells, Jurkat cells, NIH/3T3 cells, mouse brain tissue |
| Positive IHC detected in | human pancreas cancer tissue, human pancreas tissue, human skeletal muscle tissue, rat brain tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | HeLa cells |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:200-1:800 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
10155-1-AP targets CDC42 in WB, IHC, IF/ICC, IP, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.
| 经测试应用 | WB, IHC, IF/ICC, ELISA |
| 文献引用应用 | WB, IHC, IF, IP, CoIP |
| 经测试反应性 | human, mouse, rat |
| 文献引用反应性 | human, mouse, rat, chicken, bovine, hamster |
| 免疫原 |
CatNo: Ag0207 Product name: Recombinant human CDC42 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-186 aa of BC002711 Sequence: MQTIKCVVVGDGAVGKTCLLISYTTNKFPSEYVPTVFDNYAVTVMIGGEPYTLGLFDTAGQEDYDRLRPLSYPQTDVFLVCFSVVSPSSFENVKEKWVPEITHHCPKTPFLLVGTQIDLRDDPSTIEKLAKNKQKPITPETAEKLARDLKAVKYVECSALTQKGLKNVFDEAILAALEPPEPKKSR |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | cell division cycle 42 (GTP binding protein, 25kDa) |
| 别名 | EC:3.6.5.2, G25K, G25K GTP binding protein, G25K GTP-binding protein |
| 计算分子量 | 21 kDa |
| 观测分子量 | 21 kDa |
| GenBank蛋白编号 | BC002711 |
| 基因名称 | CDC42 |
| Gene ID (NCBI) | 998 |
| RRID | AB_2078096 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | P60953 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
Cdc42, the human homologue of the cell division cycle 42 of Saccharomyces cerevisiae, belongs to the Rho/Rac subfamily of Ras-like GTPases. It acts as molecular switches to regulate numerous cellular responses including the organization of the actin cytoskeleton, gene transcription, cell cycle progression, and membrane trafficking. The molecular weight is about 21 kd.
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- 作者
- 内容
- 询问日期
文献和实验| Species | Application | Title |
|---|---|---|
| human | WB,IHC | Nat Commun Spatiotemporal proteomic profiling of the pro-inflammatory response to lipopolysaccharide in the THP-1 human leukaemia cell line.Authors - Claire M Mulvey |
| mouse | WB | Circ. Res. Inhibition of KLF5-Myo9b-RhoA Pathway-Mediated Podosome Formation in Macrophages Ameliorates Abdominal Aortic Aneurysm.Authors - Dong Ma |
| human | WB | Nucleic Acids Res DOCK7 protects against replication stress by promoting RPA stability on chromatin.Authors - Ming Gao |
| human | WB | Cell Mol Life Sci Crip2 affects vascular development by fine-tuning endothelial cell aggregation and proliferationAuthors - Shuaiqi Yang |
| human | WB | Biomaterials Energy metabolism analysis reveals the mechanism of inhibition of breast cancer cell metastasis by PEG-modified graphene oxide nanosheets.Authors - Teng Zhou |
| mouse | IHC | Mol Ther Nucleic Acids miR-497 defect contributes to gastric cancer tumorigenesis and progression via regulating CDC42/ITGB1/FAK/PXN/AKT signaling.Authors - Lihui Zhang |
织细胞中广泛表达,而JNK3选择性在脑细胞中表达。 JNK/SAPK信号通路 可被应激刺激(如紫外线、热休克、高渗刺激及蛋白合成抑制剂等)、细胞因子(TNFα,IL-1)、生长因子(EGF)及某些G蛋白偶联的受体激活。外界刺激可通过Ras依赖或非Ras依赖的两条途径激活JNK,小分子G蛋白Ras超家族的成员之一Rho可能也是JNK激活的上游信号[4],Rho蛋白Rac及cdc42的作用可能是与p21激活的丝/苏氨酸激酶PAK结合,使其自身磷酸化而被激活,而活化的PAK进一步使JNK激活。已有
因此,综合来说,EarthOx的GST标签抗体 (货号#E022040)是一款同时具有高特异性、高灵敏度、多应用类型以及性价比的GST标签抗体。同时,我们也提供其他品牌的GST标签抗体,如Proteintech的GST多克隆抗体 ――GST PolyClonal Antibody(货号#10000-0-AP)以及Abcam、Santa等多种GST标签抗体产品。更多GST标签抗体以及其他如His、HA、Myc、GFP、Flag等其他标签抗体,请电话垂询。美国EarthOx品牌是一家提供高性价比免疫学和细胞
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