- ￥1350 - 3000
- Proteintech
- 10795-1-AP
- 2025年10月13日
- WB, IHC, IF/ICC, IF-P, IP, ELISA
- Rabbit
- human, mouse
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- 详细信息
- 文献和实验
- 技术资料
- 免疫原：
Recombinant human Sestrin2 protein
- 亚型：
IgG
- 保存条件：
Store at -20°C. Stable for one year after shipment.
- 克隆性：
Polyclonal
- 标记物：
Unconjugated
- 适应物种：
human, mouse
- 保质期：
详见产品包装
- 库存：
充足
- 宿主：
Rabbit
- 应用范围：
WB, IHC, IF/ICC, IF-P, IP, ELISA
- 靶点：
Sestrin 2
- 抗体英文名：
Sestrin 2 Polyclonal antibody
- 抗体名：
Sestrin 2 Polyclonal antibody
- 规格：
50ul/100ul/150ul
| 规格： | 50ul | 产品价格： | ￥1350.0 |
|---|---|---|---|
| 规格： | 100ul | 产品价格： | ￥2200.0 |
| 规格： | 150ul | 产品价格： | ￥3000.0 |
经过测试的应用
| Positive WB detected in | HEK-293 cells, human liver tissue, human brain tissue, NIH/3T3 cells, rat liver tissue, HeLa cells, K-562 cells, mouse kidney tissue, A2780 cells, RAW 264.7 cells |
| Positive IP detected in | K-562 cells |
| Positive IHC detected in | human breast cancer tissue, human ovary tumor tissue, human colon cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF-P detected in | mouse kidney tissue |
| Positive IF/ICC detected in | HeLa cells |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:2000-1:12000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:200-1:800 |
| Immunofluorescence (IF)-P | IF-P : 1:50-1:500 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
10795-1-AP targets Sestrin 2 in WB, IHC, IF/ICC, IF-P, IP, CoIP, ELISA applications and shows reactivity with human, mouse samples.
| 经测试应用 | WB, IHC, IF/ICC, IF-P, IP, ELISA |
| 文献引用应用 | WB, IHC, IF, IP, CoIP |
| 经测试反应性 | human, mouse |
| 文献引用反应性 | human, mouse, rat, pig, bovine |
| 免疫原 |
CatNo: Ag1247 Product name: Recombinant human Sestrin2 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 132-480 aa of BC013304 Sequence: HMAEFLQTGGDPEWLLGLHRAPEKLRKLSEINKLLAHRPWLITKEHIQALLKTGEHTWSLAELIQALVLLTHCHSLSSFVFGCGILPEGDADGSPAPQAPTPPSEQSSPPSRDPLNNSGGFESARDVEALMERMQQLQESLLRDEGTSQEEMESRFELEKSESLLVTPSADILEPSPHPDMLCFVEDPTFGYEDFTRRGAQAPPTFRAQDYTWEDHGYSLIQRLYPEGGQLLDEKFQAAYSLTYNTIAMHSGVDTSVLRRAIWNYIHCVFGIRYDDYDYGEVNQLLERNLKVYIKTVACYPEKTTRRMYNLFWRHFRHSEKVHVNLLLLEARMQAALLYALRAITRYMT |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | sestrin 2 |
| 别名 | SESN2, Sestrin2, SESN 2, SES2, Hypoxia-induced gene |
| 计算分子量 | 54 kDa |
| 观测分子量 | 54-60 kDa |
| GenBank蛋白编号 | BC013304 |
| 基因名称 | SESN2/Sestrin 2 |
| Gene ID (NCBI) | 83667 |
| RRID | AB_2185480 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | P58004 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
SESN is a family of highly conserved, stress-inducible genes that can defend the cell against oxidative damage and oncogenic signaling. SESN2 ,which belongs to the SESN family, has been implicated as a tumor suppressor that can inhibit angiogenesis and promote autophagy, underscoring the importance of elucidating the molecular mechanism by which SESN2 regulates pathways of metabolism and survival.(PMID:22363791). Sesn2 encodes a 480 amino acid protein which can run as a 60 kDa band on SDS-polyacrylamide gel electrophoresis(PMID:20878915).
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文献和实验| Species | Application | Title |
|---|---|---|
| human | WB | Nature Systematic quantitative analysis of ribosome inventory during nutrient stress.Authors - Heeseon An |
| mouse | WB | Nature SAR1B senses leucine levels to regulate mTORC1 signalling.Authors - Jie Chen |
| human | WB | Nature Stem cell function and stress response are controlled by protein synthesis.Authors - Sandra Blanco |
| mouse | WB | Science Zonated leucine sensing by Sestrin-mTORC1 in the liver controls the response to dietary leucine.Authors - Andrew L Cangelosi |
| human | WB | Nature SZT2 dictates GATOR control of mTORC1 signalling.Authors - Min Peng |
| mouse | WB | Cell Sestrins Function as Guanine Nucleotide Dissociation Inhibitors for Rag GTPases to Control mTORC1 Signaling.Authors - Min Peng
|
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