- ￥7180 - 14480
- 对于CRISPR基因编辑应用，我们提供传统和非整合型两种CRISPR慢病毒。对于CRISPRi基因抑制应用，我们提供的CRISPRi慢病毒套装包含2种病毒：gRNA慢病毒和dCas9-KRAB-MeCP2慢病毒。
- 广州
- 2026年01月05日
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- 详细信息
- 询价记录
- 文献和实验
- 技术资料
- 提供商：
云舟生物科技（广州）有限公司
- 服务名称：
病毒套装
- 规格：
小规格/中规格/大规格
| 规格： | 小规格 | 产品价格： | ￥7180.0 |
|---|---|---|---|
| 规格： | 中规格 | 产品价格： | ￥9480.0 |
| 规格： | 大规格 | 产品价格： | ￥14480.0 |
技术详情
当前我们提供慢病毒类型的CRISPR（3+1）和CRISPRi（3+1）套装。这些CRISPR载体系统均经过我们专门优化，在大肠杆菌中具有高拷贝复制、可包装产生高滴度病毒并且对于靶细胞具有可靠的转导效率。
我们的CRISPR慢病毒载体能将CRISPR/Cas9系统整合进细胞基因组中，随细胞分裂而分裂，实现Cas9和gRNA的长期稳定表达。如果选择非整合型慢病毒进行包装，这种慢病毒转导细胞后由于其突变的整合酶没有功能，病毒DNA不插入细胞基因组并且以非复制型游离体存在于转导细胞中，则转导进细胞的Cas9和gRNA不能持续表达，有助于降低脱靶风险。
在CRISPRi系统中，dCas9是与KRAB和MeCP2阻遏域融合在一起的，具有强大的基因阻遏功能。与shRNA相比，CRISPRi系统可以沉默更多类型基因，如蛋白编码基因、非编码RNA、microRNA等。对于我们的CRISPRi慢病毒套装，其gRNA慢病毒携带Puromycin抗性基因，而dCas9-KRAB-MeCP2慢病毒携带Hygromycin抗性基因。进行实验时，使用Puromycin和Hygromycin两种抗生素筛选转导细胞。
订购信息
注意：以上价格和周期包含载体构建
点击查看我们的慢病毒包装详情
CRISPRi（3+1）慢病毒套装
注意：以上价格和周期包含载体构建
常见问题解答
我们如何测定病毒滴度？
我应该选择单gRNA还是双gRNA用于CRISPR介导的基因敲除？
与shRNA相比，CRISPRi基因沉默具有什么特点？
如何计算gRNA特异性分值？
关注“载体家”公众号，为您详情解答。
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Life Sci Alliance. 2022. doi: 10.26508/lsa.202101240. IF: 5.781 - Enhanced Orai1-mediated store-operated Ca2+ channel/calpain signaling contributes to high glucose-induced podocyte injury
J Biol Chem. 2022. doi: 10.1016/j.jbc.2022.101990. IF: 5.486 - Stress granules and mTOR are regulated by membrane atg8ylation during lysosomal damage
J Cell Biol. 2022. doi: 10.1083/jcb.202207091. IF: 5.486 - The influence of CD26+ and CD26− fibroblasts on the regeneration of human dermo-epidermal skin substitutes
Sci Rep. 2022. doi: 10.1038/s41598-022-05309-5. IF: 4.996 - The Chd4 subunit of the NuRD complex regulates Pdx1-controlled genes involved in β-cell function
J Mol Endocrinol. 2022. doi: 10.1530/JME-22-0011. IF: 4.869 - The cytotoxic action of BCI is not dependent on its stated DUSP1 or DUSP6 targets in neuroblastoma cells
FEBS Open Bio. 2022. doi: 10.1002/2211-5463.13418. IF: 2.792 - CRISPR/Cas9 mediated approach to generate YAP-depleted human embryonic stem cell line (MUSIe002-A-1)
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Nat Commun. 2021. doi: 10.1038/s41467-021-24137-1. IF: 17.694 - Desmosomal proteins of DSC2 and PKP1 promote cancer cells survival and metastasis by increasing cluster formation in circulatory system
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Quant Imaging Med Surg. 2021. doi: 10.21037/qims-20-443. IF: 4.63 - TET2 directs mammary luminal cell differentiation and endocrine response
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Cell Syst. 2020. doi: 10.1016/j.cels.2020.06.004. IF: 11.091
157(6) (2014) 1262-1278. [5] K.H. Siu, W. Chen, Riboregulated toehold-gated gRNA for programmable CRISPR-Cas9 function, Nat Chem Biol 15(3) (2019) 217-220. [6] X.W. Wang, L.F. Hu, J. Hao, L.Q. Liao, Y.T. Chiu, M. Shi, Y. Wang, A microRNA-inducible
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