CeleMag Streptavidin Beads

cDNA Library Construction Using Streptavidin-Paramagnetic Beads and PCR

cDNA clones of genes expressed in small amounts of material can be hard to obtain because the construction of conventional cDNA libraries requires microgram amounts of poly (A)+ RNA (1 ). The polymerase chain reaction (PCR), which is commonly

Solid-Phase Sequencing of Biotinylated PCR Products with Streptavidin-Coated Magnetic Beads

during the amplification reaction. The DNA can then be immobilized onto streptavidin-coated paramagnetic beads, simultaneously removing buffers, dNTPs, and unincorporated PCR primers. By alkali denaturation, the immobilized double-stranded DNA is converted into a single

Isolation of Transcription Factor Complexes by In Vivo Biotinylation Tagging and Direct Binding to Streptavidin Beads

Efficient tagging methodologies are an integral aspect of protein complex characterization by proteomic approaches. Owing to the very high affinity of biotin for avidin and streptavidin, biotinylation tagging offers an attractive approach