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- 供应商：
上海研卉生物
- 英文名：
FastDigest HhaI
- 规格：
200 reactions
描述
| 5' | G | C | G ↓ | C | 3' | ||||
| 3' | C ↑ | G | C | G | 5' |
Thermo Scientific FastDigest HhaI restriction enzyme recognizes GCG^C site and cuts best at 37°C in 5–15 minutes using universal FastDigest Buffer. Isoschizomers: AspLEI, BstHHI, CfoI, HinP1I, HspAI.
Thermo Scientific FastDigest HhaI is one of an advanced line of fast restriction enzymes that are all 100% active in the universal FastDigest and FastDigest Green reaction buffers.
The universal buffer allows rapid single-, double-, or multiple DNA digestion within 5–15 minutes eliminating any need for buffer change or subsequent DNA clean-up steps. See Reaction Conditions for FastDigest Enzymes for a table of enzyme activity, heat inactivation and incubation times for this and other FastDigest restriction enzymes. DNA modifying enzymes, such as Klenow Fragment, T4 DNA Ligase, alkaline phosphatases and T4 DNA Polymerase all have 100% activity in FastDigest Buffer. Therefore, enzymes for downstream applications can be directly added to the FastDigest reaction mix.
For additional convenience FastDigest Green Buffer includes a density reagent and two tracking dyes for direct loading of digestion reaction products on gels.
Short incubation times and optimal composition of the universal FastDigest Buffer eliminate star activity effects.
Features
• 100% activity of all FastDigest enzymes in the universal buffer
• 100% buffer compatibility with downstream applications
规格
| Compatible Buffer: | 10x FastDigest Buffer/FastDigest Green Buffer |
|---|---|
| Enzyme: | HhaI |
| Methylation Sensitivity: | CpG methylation-sensitive, Not dam methylation-sensitive, Not dcm methylation-sensitive |
| Optimal Reaction Temperature: | 37° C |
| Product Line: | FastDigest |
| Product Size: | 200 reactions |
| Sensitive to Heat Inactivation: | No |
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the fragment with Eco47III, HaeII, or HhaI. (FYI-HaeII and HhaI are much less expensive than Eco47III.) The “d” allele does not cut with the enzyme due to a polymorphism at that site, but the b1 allele cuts once to yield 142bp and 76bp fragments. The Ahr
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and can assist in interpretation of allele patterns. 4. References Allen RC, Zoghbi HY, Moseley AB, Rosenblatt HM, Belmont JW. Methylation of HpaII and HhaI sites near the polymorphic CAG repeat in the human androgen-receptor gene correlates with X chromosome
Denaturing Gradient Gel Electrophoresis (DGGE)
(which may occur as frequently as every 400 bp). Genomic DNA is digested with an enzyme combination that cuts the DNA into fragments of average size 200-700 bp. Enzymes with 4-base recognition sites, such as AluI, HaeIII, HhaI, MspI, and RsaI are the most useful
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