- ￥7434.84
- SBI(systembio)
- TMEXO-1
- 2026年01月07日
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- 详细信息
- 文献和实验
- 技术资料
- 库存：
6
- 英文名：
Thrombin Plasma Prep
- 供应商：
上海觅拓生物科技有限公司
- 保存条件：
Dry Ice
- 规格：
500 ul
Overview
Efficient exosome isolation from plasma
“We therefore pursued the ExoQuick® method for further study, as these samples required much less sample input, a key benefit when working with clinical samples and mouse models1.”
When isolating exosomes from plasma, fibrinogen and fibrin can impede efficient recovery. By pre-treating plasma with thrombin, the fibrinogen can be converted to fibrin and easily pre-cleared by a simple centrifugation step. The resulting serum-like solution can now be treated with ExoQuick, original formulation, for quantitative exosome isolation that is compatible with high-throughput methods.
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文献和实验tests of haemostasis in blood or plasma samples are simple endpoint measures of the potential to generate thrombin. Thus, there has been a recent surge towards direct measurement of thrombin generation potential in plasma/blood samples as a refined
, prior to performing additional coagulation assays. This test is based on the principle that in citrated plasma, the addition of Thrombin allows for formation of a stable clot. The time required for the formation of a stable clot is recorded in seconds
, or freeze as is. Save 100 ul of unconcentrated, purified protein. Load 1-10 ul for SDS-PAGE. Quantitate by comparison to 2.5, 1.0, and 0.25mg BSA standards on the same gel. BINDING TO BEADS AND THROMBIN CLEAVAGE Alternate Mini Prep
