Rat Primary Brain Microvascula

Purification of Endothelial Cells from Rat Brain

Endothelial cells make up a minor population of cells in a tissue, but play a major role in tissue homeostasis, as well as in diverse pathologies. To understand the biology of cerebral endothelium, purification and characterization

Generation of Primary Cultures of Bovine Brain Endothelial Cells and Setup of Cocultures with Rat Astrocytes

to reflect the tightness of the in vivo blood–brain barrier. The present protocol describes the setup of an in vitro coculture model based on primary cultures of endothelial cells from bovine brain microvessels and primary cultures of rat astrocytes

Isolation and Culture of Microvascular Endothelial Cells

. However, there is convincing evidence that microvascular endothelial cells display a number of important functional differences, compared to large vessel-derived endothelial cells (3 ), in particular, with regard to their growth factor response (4 ,5 ) and their regulation