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- Gibco
- 12558011
- 2025年09月29日
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- 详细信息
- 文献和实验
- 技术资料
- 库存：
100
- 供应商：
上海觅拓
- 英文名：
AmnioMAX™ C-100 Complete Medium
- 规格：
1 set
Basal Medium:
Storage: 2 – 8°C
Ships: Ambient temperature
Shelf life: 16 months from date of manufacture
Supplement:
Storage: -5 to -20°C
Ships: Frozen
Shelf life: 16 months from date of manufacture
Gibco® AmnioMAX™ C-100 Complete Medium is a kit used to prepare a fully-supplemented medium developed for the short term culture of human amniotic fluid cells for cytogenetic studies and in vitro diagnostic procedures.
Gibco® AmnioMAX™ C-100 Complete Medium features:
• Easy-to-use format
• Quality and performance testing
• Unique, optimized formulation
Easy-to-use format
Gibco® AmnioMAX™ C-100 Complete Medium is a two-part kit for preparation of a complete medium that requires no additional supplementation. The complete medium can be stored at 2–8°C for up to ten days.
Quality and performance testing
Every lot of Gibco® AmnioMAX™ C-100 Complete Medium is performance tested by a certified US reference cytogenetics laboratory to ensure consistently superior performance. Pooled primary human amniotic fluid samples are cultured for six days in Gibco® AmnioMAX™ C-100 Complete Medium before measuring the total number of colonies and total number of mitotic colonies. In addition, each lot is tested for sterility, pH and osmolality.
Unique, optimized formulation
Gibco® AmnioMAX™ C-100 Complete Medium contains Fetal Bovine Serum (FBS), gentamicin, and L-glutamine to maximize cell attachment and growth. This optimized medium also has an enhanced buffering system that provides greater pH stability during culture manipulations.
Product Use
For in vitro diagnostic use.
cGMP Manufacturing and Quality System
Gibco® AmnioMAX™ C-100 Complete Medium is manufactured at a cGMP compliant facility, located in Grand Island, New York. The facility is registered with the FDA as a medical device manufacturer and is certified to ISO 13485 and ISO 9001 standards.
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文献和实验指培养细胞时，具备可使其最大限度地生长和繁殖的条件，而含有满足各种营养缺陷突变型要求的培养基。其组成可按生物种类和目的而异，但一般是由酵母浸膏、麦芽汁、牛肉膏、蛋白胨、胰蛋白胨、酪蛋白氨基酸等营养物中选几种营养物配合而成。完全培养基用于短期内无性增殖大量细胞，以及分离和增殖营养缺陷突变型。
unsuccessful, emphasizing a need for further refinement. A novel method, the complete open reading frame (C-ORF) technique, is presently described, which has proven successful in cases, where standard rapid amplification of cDNA ends (RACE) has not worked. In C
Polymerase - has 3'' to 5'' exonuclease, but not 5'' to 3'' exonuclease activity. Its half-life at 97.5°C is 40-50 minutes. 10X Buffer from Perkin-Elmer for this enzyme is 100 mM Tris-HCl, pH 8.8, 100 mM KCl, and 0.02% tween 20. It is used at 2-6 U/100 µl
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