Recombinant Mouse M-CSF/CSF1 (CB34)
产品说明(Description)
Recombinant Mouse Macrophage colony-stimulating factor 1 is produced by our Mammalian expression system and the target gene encoding Lys33-Glu262 is expressed.
Accession #: P07141
Known as: Macrophage colony-stimulating factor 1;CSF-1;MCSF;Csf1;Csfm
制剂(Formulation)
Lyophilized from a 0.2 μm filtered solution of PBS, pH7.4.
质量控制(Quality Control)
Purity: Greater than 95% as determined by reducing SDS-PAGE.
Endotoxin: Less than 0.1 ng/ug (1 EU/ug) as determined by LAL test.
Bioactivity: Measured in a cell proliferation assay using M-NFS-60 mouse myelogenous leukemia lymphoblast cells.
The ED50 for this effect is 0.04-0.2 ng/mL.
复溶(Reconstitution)
Always centrifuge tubes before opening. Do not mix by vortex or pipetting.
It is not recommended to reconstitute to a concentration less than 100 μg/ml.
Dissolve the lyophilized protein in distilled water.
Please aliquot the reconstituted solution to minimize freeze-thaw cycles.
保存(Storage)
Lyophilized protein should be stored at < -20°C, though stable at room temperature for 3 weeks.Reconstituted protein solution can be stored at 4-7°C for 2-7 days.Aliquots of reconstituted samples are stable at < -20°C for 3 months.
背景(Background)
Macrophage colony-stimulating factor 1(M-csf)is a single-pass type I membrane protein . It is a hematopoietic growth factor that is involved in the proliferation, differentiation, and survival of monocytes, macrophages, and bone marrow progenitor cells. M-CSF affects macrophages and monocytes in several ways, including stimulating increased phagocytic and chemotactic activity, and increased tumour cell cytotoxicity. The role of M-CSF is not only restricted to the monocyte/macrophage cell lineage. By interacting with its membrane receptor, M-CSF also modulates the proliferation of earlier hematopoietic progenitors and influence numerous physiological processes involved in immunology, metabolism, fertility and pregnancy.
电泳(SDS-PAGE)
参考文献(References)
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The extract of Trachelospermum jasminoides (Lindl.) Lem. vines inhibits osteoclast differentiation through the NF-κB, MAPK and AKT signaling pathways[J]. Tao Jiang. et al. Biomedicine & Pharmacotherapy. 2020.
IGFBP7 acts as a negative regulator of RANKL-induced osteoclastogenesis and oestrogen deficiency-induced bone loss[J]. Chenyi Ye. et al. Cell Proliferation. 2019.
17β-Estradiol promotes LC3B-associated phagocytosis in trained immunity of female mice against sepsis[J]. Zhiheng Sun. et al. International Journal of Biological Sciences. 2021.
Histone Hyperacetylation Mediates Enhanced IL-1β Production in LPS/IFN-γ stimulated Macrophages[J]. Zhen Dong. et al. IMMUNOLOGY. 2020.
Myeloid-specific SIRT1 deletion exacerbates airway inflammatory response in a mouse model of allergic asthma[J]. Tianwen Lai. et al. Aging-US. 2021.
Corilagin suppresses RANKL‐induced osteoclastogenesis and inhibits oestrogen deficiency‐induced bone loss via the NF‐κB and PI3K/AKT signalling pathways[J]. Jinwei Lu. et al. JOURNAL OF CELLULAR AND MOLECULAR MEDICINE. 2020.
Identification of compound D2923 as a novel anti-tumor agent targeting CSF1R[J]. Ying-Qiang Liu. et al. Acta Pharmacologica Sinica. 2018.
The prevention of latanoprost on osteoclastgenesis in vitro and lipopolysaccharide‐induced murine calvaria osteolysis in vivo[J]. Xing Xu. et al. Journal of Cellular Biochemistry. 2017.
FOR RESEARCH USE ONLY